Detailed information of TA system TA182821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1333279..1334867
• Replicon: chromosome
• Accession: NZ_CP065467
• Organism: Klebsiella quasipneumoniae strain Pinhead Larry

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: I5M56_RS06425
• Protein ID: WP_194425825.1
• Coordinates: 1333279..1334601 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: I5M56_RS06430
• Protein ID: WP_004205921.1
• Coordinates: 1334601..1334867 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M56_RS06390	1328361..1328579	+	219	WP_002904808.1	ParD-like family protein	-
I5M56_RS06395	1328560..1329342	+	783	WP_049003659.1	type I methionyl aminopeptidase	-
I5M56_RS06400	1329611..1330726	+	1116	WP_108450436.1	serine hydrolase	-
I5M56_RS06405	1330713..1331057	+	345	WP_108450437.1	hypothetical protein	-
I5M56_RS06410	1331086..1331619	+	534	WP_108450438.1	DUF2975 domain-containing protein	-
I5M56_RS06415	1331622..1331828	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
I5M56_RS06420	1331970..1333262	+	1293	WP_023318214.1	glycoside hydrolase family 10 protein	-
I5M56_RS06425	1333279..1334601	-	1323	WP_194425825.1	type II toxin-antitoxin system HipA family toxin	Toxin
I5M56_RS06430	1334601..1334867	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I5M56_RS06435	1335150..1335476	-	327	WP_108450439.1	hydroxyisourate hydrolase	-
I5M56_RS06440	1335473..1335973	-	501	WP_017900378.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
I5M56_RS06445	1335951..1337330	-	1380	WP_017900377.1	purine permease	-
I5M56_RS06450	1337676..1338830	+	1155	WP_004205928.1	FAD-dependent urate hydroxylase HpxO	-
I5M56_RS06455	1338811..1339734	-	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48899.16 Da
• Isoelectric Point: 7.8920

>T182821 WP_194425825.1 NZ_CP065467:c1334601-1333279 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILGEFADRVP
QAIESARQTLPSDFSAHVWRAITENMLKLHARLQQGLLAG

Nucleotide

• Length: 1323 bp

>T182821 NZ_CP086604:3926701-3927015 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT182821 WP_004205921.1 NZ_CP065467:c1334867-1334601 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT182821 NZ_CP086604:3926465-3926698 [Escherichia fergusonii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9