Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 25802..26445 Replicon plasmid pOlaf_02
Accession NZ_CP065466
Organism Klebsiella pneumoniae strain Olaf

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag I5M57_RS28145 Protein ID WP_001044770.1
Coordinates 26029..26445 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag I5M57_RS28140 Protein ID WP_001261282.1
Coordinates 25802..26032 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M57_RS28105 21025..21804 - 780 WP_013214009.1 site-specific integrase -
I5M57_RS28110 21988..22992 - 1005 WP_011977814.1 hypothetical protein -
I5M57_RS28115 23022..23225 - 204 WP_011977813.1 hypothetical protein -
I5M57_RS28120 23271..23792 - 522 WP_013214008.1 hypothetical protein -
I5M57_RS28125 23850..24242 - 393 WP_011977811.1 hypothetical protein -
I5M57_RS28130 24279..25223 - 945 WP_011977810.1 hypothetical protein -
I5M57_RS28135 25384..25845 - 462 WP_014343465.1 hypothetical protein -
I5M57_RS28140 25802..26032 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I5M57_RS28145 26029..26445 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
I5M57_RS28150 26519..28081 + 1563 WP_004206609.1 AAA family ATPase -
I5M57_RS28155 28066..29088 + 1023 WP_000361404.1 helicase UvrD -
I5M57_RS28160 29344..30041 + 698 Protein_36 IS1 family transposase -
I5M57_RS28165 30070..30342 + 273 Protein_37 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 / blaCTX-M-65 / tet(A) - 1..105091 105091


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T182808 WP_001044770.1 NZ_CP065466:26029-26445 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T182808 NZ_CP086599:c309440-309129 [Nocardia farcinica]
GTGAACGCGCCTGTACGTGGCCAGGTCTACCGGGCGGATCTGGGCTACGGGCCCAAGCCCTGGTTGATCGTGTCCAACAA
CCAGCGCAACCGCGTGACCTTGGACGTGCTCGCGGTTCGGATCACCACCACCCGGCGAGAGCTGCCCACCTGGGTGGAGC
TGGGATCCAATGAGCCGCTGACCGGTTTCGTCAATACGGACAACATCGAGACTCTGGCCAAGGATGAGCTCGGCGACTAC
CTGGGTTCGCTCTCTGCGAGCGCGATCTTGGAAGTCAACCGCGCGCTCACCATCGCGCTCGGCATTCCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT182808 WP_001261282.1 NZ_CP065466:25802-26032 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT182808 NZ_CP086599:c309691-309437 [Nocardia farcinica]
ATGACACATGCGATGACCGTCAGATTGGACGACGAGACGTTCCGCCGTCTCCAAGAGCTGGAGCAGGCAGGCGCTCCCTC
GCGGTCAGCGGCAGTGGTGGCAGCAATCCACGAGGCGTGGAACCGGCTCCAGGATGAGCAGTTGGCCCGAGCCTACGAGG
CGGCAGTAGCCCAGTCGCCCACCTACCCGTACGAGGACGAGGACGAGCGCGCCGTACTGCGTGCCCGGCGTAACAAGCGC
CAGATCCCCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References