Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5407309..5407934 Replicon chromosome
Accession NZ_CP065464
Organism Klebsiella pneumoniae strain Olaf

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I5M57_RS26725 Protein ID WP_002882817.1
Coordinates 5407551..5407934 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I5M57_RS26720 Protein ID WP_004150355.1
Coordinates 5407309..5407551 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M57_RS26695 5403300..5403899 + 600 WP_004151865.1 glucose-1-phosphatase -
I5M57_RS26700 5403893..5404753 + 861 WP_002882807.1 virulence factor BrkB family protein -
I5M57_RS26705 5404750..5405187 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I5M57_RS26710 5405232..5406173 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I5M57_RS26715 5406187..5407104 - 918 WP_002882812.1 alpha/beta hydrolase -
I5M57_RS26720 5407309..5407551 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I5M57_RS26725 5407551..5407934 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I5M57_RS26730 5408108..5409037 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I5M57_RS26735 5409034..5409669 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I5M57_RS26740 5409666..5410568 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182806 WP_002882817.1 NZ_CP065464:5407551-5407934 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182806 NZ_CP086594:c2662345-2662079 [Sphingomonas radiodurans]
GTGATCCTGACGTTTTCGTCGCGTGCCTGGTCCGAGTATCTTGAATGGCAGGGTGACGACCGGCGAGGTCTGGAGCGCGT
CAACGCGTTGATCAAGGAGTGCCAGCGCGATCCGTTCCGCGGCACCGGCAAGCCAGAGCCGCTTGGCGGTAATTTATCTG
GCTGGTGGTCCCGCCGCATCAATCGCGAGCATCGGCTGGTGTATCGGGTGAGCGGTGCCGGCGACGCGCAGGCGCTGGAG
ATCGCGCAGTGCCGGTATCATTATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182806 WP_004150355.1 NZ_CP065464:5407309-5407551 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182806 NZ_CP086594:c2662596-2662342 [Sphingomonas radiodurans]
ATGCAGTCGCTCAGCATCTCCGAAGCCCGCGCCAATATGAAAGCGCTCGTGGACCGCGTGGTCGCCGACAAAGCGCCGGT
GTCGATCACCCGTCAGAAGGGCGAGGGCGTGGTAATGATCTCGCAAAGCGAATGGTCGTCGATCGAGGAGACGCTCTATC
TCCTGCAATCGCCGGCGAACGCGAAGCGCCTTCTGGAAAGCATCGGCGAGCTTGATGCCGGGGCGGGCGTGGAGCGCGCG
CTGATCGAACCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References