Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20585..21228 Replicon plasmid pGordon_01
Accession NZ_CP065461
Organism Klebsiella pneumoniae strain Gordon

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag I5M58_RS27550 Protein ID WP_001044770.1
Coordinates 20812..21228 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag I5M58_RS27545 Protein ID WP_001261282.1
Coordinates 20585..20815 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M58_RS27510 15808..16587 - 780 WP_013214009.1 site-specific integrase -
I5M58_RS27515 16771..17775 - 1005 WP_011977814.1 hypothetical protein -
I5M58_RS27520 17805..18008 - 204 WP_011977813.1 hypothetical protein -
I5M58_RS27525 18054..18575 - 522 WP_013214008.1 hypothetical protein -
I5M58_RS27530 18633..19025 - 393 WP_011977811.1 hypothetical protein -
I5M58_RS27535 19062..20006 - 945 WP_011977810.1 hypothetical protein -
I5M58_RS27540 20167..20628 - 462 WP_014343465.1 hypothetical protein -
I5M58_RS27545 20585..20815 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I5M58_RS27550 20812..21228 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
I5M58_RS27555 21302..22864 + 1563 WP_004206609.1 AAA family ATPase -
I5M58_RS27560 22849..23871 + 1023 WP_000361404.1 helicase UvrD -
I5M58_RS27565 24127..24824 + 698 Protein_29 IS1 family transposase -
I5M58_RS27570 24853..25125 + 273 Protein_30 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 / blaCTX-M-65 / tet(A) - 1..105090 105090


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T182782 WP_001044770.1 NZ_CP065461:20812-21228 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T182782 NZ_CP086572:1183811-1183999 [Staphylococcus argenteus]
ATGACTTTACATTTTGCAATTTTATTTTGGCTAGCATTAATTTTTATCGTTGCTGGTACATTTATATTTGTCTTAATGAA
AAAAACTGCTAAAGAATCTAAAAAAGAATCTTATTTAAGCTTCGCTGTTATTTTCTATATTTTTGGATTCGCAATGCTAA
TTTATACATTAATATTTGGAATTCTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT182782 WP_001261282.1 NZ_CP065461:20585-20815 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT182782 NZ_CP086572:1183522-1183782 [Staphylococcus argenteus]
ATGAAAAACATTCAATTAAATGCTATAAATTTAGTTATAACAATCATATTTGCTATCTTTAACATTATGATTACATATAA
CAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGCATTATCATTTGCGGTTCGATACTCATTGTATCGTTTACCATTG
CAATGATTACTAAATTCTGGTTAAGTGAAATATTATTTTTTATTAACATCGTGCTCGTTCTTTATTATATTTATCCAATT
TTTTATGATTTCATTAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References