Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 5120330..5121140 Replicon chromosome
Accession NZ_CP065460
Organism Klebsiella pneumoniae strain Gordon

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag I5M58_RS25425 Protein ID WP_002887280.1
Coordinates 5120330..5120863 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag I5M58_RS25430 Protein ID WP_002887278.1
Coordinates 5120874..5121140 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M58_RS25420 5119161..5120282 + 1122 WP_002887282.1 cupin domain-containing protein -
I5M58_RS25425 5120330..5120863 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
I5M58_RS25430 5120874..5121140 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
I5M58_RS25435 5121243..5122676 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
I5M58_RS25440 5122666..5123349 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
I5M58_RS25445 5123521..5124906 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
I5M58_RS25450 5124924..5125268 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T182779 WP_002887280.1 NZ_CP065460:c5120863-5120330 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T182779 NZ_CP086570:2692359-2692721 [Staphylococcus argenteus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTTGT
CATTATTCAAAATGACACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCCA
AAATACCGACCCATGTAGAGATTGAAAAGAAAAAATATAAACTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGG
ACACTTGATAAAAAACGTTTAAAAGAAAAACTGACGTACTTATCAGACAACAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAACACAATGGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT182779 WP_002887278.1 NZ_CP065460:c5121140-5120874 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT182779 NZ_CP086570:2692192-2692362 [Staphylococcus argenteus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCAATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References