Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 168612..169237 Replicon chromosome
Accession NZ_CP065460
Organism Klebsiella pneumoniae strain Gordon

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I5M58_RS00805 Protein ID WP_002882817.1
Coordinates 168612..168995 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I5M58_RS00810 Protein ID WP_004150355.1
Coordinates 168995..169237 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M58_RS00790 165978..166880 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I5M58_RS00795 166877..167512 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I5M58_RS00800 167509..168438 + 930 WP_194315501.1 formate dehydrogenase accessory protein FdhE -
I5M58_RS00805 168612..168995 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I5M58_RS00810 168995..169237 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I5M58_RS00815 169442..170359 + 918 WP_002882812.1 alpha/beta hydrolase -
I5M58_RS00820 170373..171314 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I5M58_RS00825 171359..171796 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I5M58_RS00830 171793..172653 - 861 WP_002882807.1 virulence factor BrkB family protein -
I5M58_RS00835 172647..173246 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182764 WP_002882817.1 NZ_CP065460:c168995-168612 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182764 NZ_CP086566:c309196-309101 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182764 WP_004150355.1 NZ_CP065460:c169237-168995 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182764 NZ_CP086566:309002-309066 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References