Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1005966..1006585 Replicon chromosome
Accession NZ_CP065457
Organism Klebsiella pneumoniae strain Magic conch shell

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I5M59_RS04820 Protein ID WP_002892050.1
Coordinates 1005966..1006184 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I5M59_RS04825 Protein ID WP_002892066.1
Coordinates 1006211..1006585 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M59_RS04785 1002013..1002276 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
I5M59_RS04790 1002276..1002416 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I5M59_RS04795 1002413..1003111 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
I5M59_RS04800 1003212..1004663 + 1452 WP_023302231.1 PLP-dependent aminotransferase family protein -
I5M59_RS04805 1004638..1005108 - 471 WP_002892026.1 YlaC family protein -
I5M59_RS04810 1005129..1005269 + 141 WP_004147370.1 hypothetical protein -
I5M59_RS04815 1005241..1005807 - 567 WP_002892030.1 maltose O-acetyltransferase -
I5M59_RS04820 1005966..1006184 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I5M59_RS04825 1006211..1006585 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I5M59_RS04830 1007071..1010217 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
I5M59_RS04835 1010240..1011433 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182747 WP_002892050.1 NZ_CP065457:c1006184-1005966 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T182747 NZ_CP086560:c26858-26517 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT182747 WP_002892066.1 NZ_CP065457:c1006585-1006211 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT182747 NZ_CP086560:c27088-26858 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References