Detailed information of TA system TA182746

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 817287..817993
• Replicon: chromosome
• Accession: NZ_CP065457
• Organism: Klebsiella pneumoniae strain Magic conch shell

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: I5M59_RS03825
• Protein ID: WP_024622903.1
• Coordinates: 817287..817655 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A485WAM6
• Locus tag: I5M59_RS03830
• Protein ID: WP_023302278.1
• Coordinates: 817676..817993 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M59_RS03810	812848..813807	-	960	WP_023302280.1	thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha	-
I5M59_RS03815	814181..816826	+	2646	WP_024622905.1	LuxR family transcriptional regulator	-
I5M59_RS03820	816949..817290	-	342	WP_024622904.1	hypothetical protein	-
I5M59_RS03825	817287..817655	-	369	WP_024622903.1	TA system toxin CbtA family protein	Toxin
I5M59_RS03830	817676..817993	-	318	WP_023302278.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I5M59_RS03835	818011..818232	-	222	WP_023302277.1	DUF987 domain-containing protein	-
I5M59_RS03840	818241..818723	-	483	WP_023302276.1	RadC family protein	-
I5M59_RS03845	818732..819190	-	459	WP_023302275.1	antirestriction protein	-
I5M59_RS03850	819277..819513	-	237	WP_032410024.1	DUF905 domain-containing protein	-
I5M59_RS03855	819591..820001	-	411	WP_023302274.1	hypothetical protein	-
I5M59_RS03860	820068..820505	-	438	WP_023301392.1	hypothetical protein	-
I5M59_RS03865	820547..821083	-	537	WP_023302273.1	DUF4339 domain-containing protein	-
I5M59_RS03870	821109..821819	-	711	WP_023302272.1	DeoR family transcriptional regulator	-
I5M59_RS03875	822028..822852	-	825	WP_023302271.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.82 Da
• Isoelectric Point: 7.2897

>T182746 WP_024622903.1 NZ_CP065457:c817655-817287 [Klebsiella pneumoniae]
MKNLPATISQAAKPCLSPVAVWQMLLTHLLEQHYGLMLSDTPFSDEAVIQEHIDAGITLANAVNFLVEKYELVRIDRCGF
SSQVQAPYLTATDILHARKACGLMSRYSYREVSNIVLSRSRI

Nucleotide

• Length: 369 bp

>T182746 NZ_CP086558:2982373-2982537 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAACTATCGTTTATTGTACAACATATGAAACAATTCAGACGATCCTTGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAGAAGACAACAGAAATGACCGCTGTTTAACTTTGGCTA
GTTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11860.67 Da
• Isoelectric Point: 6.4629

>AT182746 WP_023302278.1 NZ_CP065457:c817993-817676 [Klebsiella pneumoniae]
MSNPTWGLLRDITPCLGARLVQEGNRLYYLVDRASITGKFSDAEILKLDVVFPHFISQMESMLTIGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYLTQR

Nucleotide

• Length: 318 bp

>AT182746 NZ_CP086558:c2982641-2982446 [Enterococcus faecalis]
TGTGCTGCAATAGAAACGAAAAGAGAGGTATGCTTCCAACATAGCTCTCTAATGTAGAACCGTTACTAACGGTGACAAAA
TTTTAAAGATTTTGAAATGACCGTCTAACTAGCCAAAGTTAAACAGCGGTCATTTCTGTTGTCTTCTTTAAGCAATTTCA
CAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Structures

Antitoxin

• AlphaFold DB: A0A485WAM6