Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 379216..380026 Replicon chromosome
Accession NZ_CP065457
Organism Klebsiella pneumoniae strain Magic conch shell

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag I5M59_RS01775 Protein ID WP_004178461.1
Coordinates 379493..380026 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag I5M59_RS01770 Protein ID WP_002887278.1
Coordinates 379216..379482 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M59_RS01750 375088..375432 - 345 WP_004222368.1 cation efflux system protein CusF -
I5M59_RS01755 375450..376835 - 1386 WP_048255467.1 efflux transporter outer membrane subunit -
I5M59_RS01760 377007..377690 + 684 WP_004214143.1 copper response regulator transcription factor CusR -
I5M59_RS01765 377680..379113 + 1434 WP_048255468.1 Cu(+)/Ag(+) sensor histidine kinase -
I5M59_RS01770 379216..379482 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
I5M59_RS01775 379493..380026 + 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
I5M59_RS01780 380074..381195 - 1122 WP_004186850.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T182745 WP_004178461.1 NZ_CP065457:379493-380026 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T182745 NZ_CP086558:2982373-2982537 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAACTATCGTTTATTGTACAACATATGAAACAATTCAGACGATCCTTGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAGAAGACAACAGAAATGACCGCTGTTTAACTTTGGCTA
GTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT182745 WP_002887278.1 NZ_CP065457:379216-379482 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT182745 NZ_CP086558:c2982371-2982186 [Enterococcus faecalis]
TGCTACAATAGAAACGAAAAGAGAGGTATGCTGTAACATACCTCTCTAATGTAGAACCGTTTAAGACGGTGACCATGTTA
ATTATTTAAAAATAACCGTACTCGGTCAAAGTAGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGCGC
AATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References