182735

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2824777..2825402 Replicon chromosome
Accession NZ_CP065453
Organism Klebsiella pneumoniae strain Beach Ranger

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I5M60_RS14010 Protein ID WP_002882817.1
Coordinates 2825019..2825402 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I5M60_RS14005 Protein ID WP_004150355.1
Coordinates 2824777..2825019 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M60_RS13980 2820768..2821367 + 600 WP_004151865.1 glucose-1-phosphatase -
I5M60_RS13985 2821361..2822221 + 861 WP_002882807.1 virulence factor BrkB family protein -
I5M60_RS13990 2822218..2822655 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I5M60_RS13995 2822700..2823641 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I5M60_RS14000 2823655..2824572 - 918 WP_108714542.1 alpha/beta hydrolase -
I5M60_RS14005 2824777..2825019 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I5M60_RS14010 2825019..2825402 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I5M60_RS14015 2825576..2826505 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I5M60_RS14020 2826502..2827137 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I5M60_RS14025 2827134..2828036 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182735 WP_002882817.1 NZ_CP065453:2825019-2825402 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182735 NZ_CP086558:c348897-348802 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGCTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182735 WP_004150355.1 NZ_CP065453:2824777-2825019 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182735 NZ_CP086558:348703-348767 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGCAGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References