Detailed information of TA system TA182721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 7384..8120
• Replicon: plasmid pOceanRanger_05
• Accession: NZ_CP065449
• Organism: Klebsiella pneumoniae strain Ocean Ranger

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I5M61_RS27620
• Protein ID: WP_003026803.1
• Coordinates: 7638..8120 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I5M61_RS27615
• Protein ID: WP_003026799.1
• Coordinates: 7384..7650 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M61_RS27570	3446..3808	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
I5M61_RS27575	3858..4208	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
I5M61_RS27580	4566..4835	+	270	WP_004152102.1	hypothetical protein	-
I5M61_RS27585	4823..5398	+	576	WP_004152103.1	hypothetical protein	-
I5M61_RS27590	5429..5923	+	495	WP_004152104.1	DNA-binding protein	-
I5M61_RS27595	5967..6335	+	369	WP_004152105.1	hypothetical protein	-
I5M61_RS27600	6369..6572	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
I5M61_RS27605	6621..6878	+	258	WP_004152107.1	hypothetical protein	-
I5M61_RS27610	6954..7208	+	255	WP_004152108.1	hypothetical protein	-
I5M61_RS27615	7384..7650	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I5M61_RS27620	7638..8120	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I5M61_RS27625	8321..9724	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
I5M61_RS27630	9753..10385	-	633	WP_001567369.1	hypothetical protein	-
I5M61_RS27635	10820..11824	-	1005	Protein_16	IS110 family transposase	-
I5M61_RS27640	11903..12460	-	558	WP_003100847.1	recombinase family protein	-
I5M61_RS27645	12454..12825	-	372	WP_003100853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaDHA-1 / qnrB4 / catA1	-	1..150314	150314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T182721 WP_003026803.1 NZ_CP065449:7638-8120 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T182721 NZ_CP086556:1855467-1855718 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT182721 WP_003026799.1 NZ_CP065449:7384-7650 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT182721 NZ_CP086556:1855715-1855969 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66