Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 84710..85353 Replicon plasmid pSeaSuperman_01
Accession NZ_CP065445
Organism Klebsiella pneumoniae strain Sea Superman

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag I5M62_RS27685 Protein ID WP_001044770.1
Coordinates 84710..85126 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag I5M62_RS27690 Protein ID WP_001261282.1
Coordinates 85123..85353 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M62_RS27665 80813..81085 - 273 Protein_111 transposase -
I5M62_RS27670 81114..81811 - 698 Protein_112 IS1 family transposase -
I5M62_RS27675 82067..83089 - 1023 WP_000361404.1 helicase UvrD -
I5M62_RS27680 83074..84636 - 1563 WP_004206609.1 AAA family ATPase -
I5M62_RS27685 84710..85126 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
I5M62_RS27690 85123..85353 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I5M62_RS27695 85310..85771 + 462 WP_194315298.1 hypothetical protein -
I5M62_RS27700 85932..86876 + 945 WP_011977810.1 hypothetical protein -
I5M62_RS27705 86913..87305 + 393 WP_011977811.1 hypothetical protein -
I5M62_RS27710 87363..87884 + 522 WP_013214008.1 hypothetical protein -
I5M62_RS27715 87930..88133 + 204 WP_011977813.1 hypothetical protein -
I5M62_RS27720 88163..89167 + 1005 WP_011977814.1 hypothetical protein -
I5M62_RS27725 89351..90130 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..94245 94245


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T182701 WP_001044770.1 NZ_CP065445:c85126-84710 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T182701 NZ_CP086544:901400-901807 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT182701 WP_001261282.1 NZ_CP065445:c85353-85123 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT182701 NZ_CP086544:901153-901419 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References