Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5269671..5270296 Replicon chromosome
Accession NZ_CP065444
Organism Klebsiella pneumoniae strain Sea Superman

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I5M62_RS26080 Protein ID WP_002882817.1
Coordinates 5269913..5270296 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I5M62_RS26075 Protein ID WP_004150355.1
Coordinates 5269671..5269913 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M62_RS26050 5265662..5266261 + 600 WP_004151865.1 glucose-1-phosphatase -
I5M62_RS26055 5266255..5267115 + 861 WP_002882807.1 virulence factor BrkB family protein -
I5M62_RS26060 5267112..5267549 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I5M62_RS26065 5267594..5268535 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I5M62_RS26070 5268549..5269466 - 918 WP_002882812.1 alpha/beta hydrolase -
I5M62_RS26075 5269671..5269913 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I5M62_RS26080 5269913..5270296 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I5M62_RS26085 5270470..5271399 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I5M62_RS26090 5271396..5272031 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I5M62_RS26095 5272028..5272930 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182696 WP_002882817.1 NZ_CP065444:5269913-5270296 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182696 NZ_CP086541:1639-1890 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATCACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGAAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182696 WP_004150355.1 NZ_CP065444:5269671-5269913 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182696 NZ_CP086541:1877-2104 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References