Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3071879..3072504 Replicon chromosome
Accession NZ_CP065441
Organism Klebsiella pneumoniae strain Patrick Star

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I5M63_RS15155 Protein ID WP_002882817.1
Coordinates 3071879..3072262 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I5M63_RS15160 Protein ID WP_004150355.1
Coordinates 3072262..3072504 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M63_RS15140 3069245..3070147 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I5M63_RS15145 3070144..3070779 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I5M63_RS15150 3070776..3071705 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I5M63_RS15155 3071879..3072262 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I5M63_RS15160 3072262..3072504 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I5M63_RS15165 3072709..3073626 + 918 WP_002882812.1 alpha/beta hydrolase -
I5M63_RS15170 3073640..3074581 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I5M63_RS15175 3074626..3075063 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I5M63_RS15180 3075060..3075920 - 861 WP_002882807.1 virulence factor BrkB family protein -
I5M63_RS15185 3075914..3076513 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182665 WP_002882817.1 NZ_CP065441:c3072262-3071879 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182665 NZ_CP086532:c654209-653745 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182665 WP_004150355.1 NZ_CP065441:c3072504-3072262 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182665 NZ_CP086532:c654544-654209 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References