Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 452525..454113 Replicon chromosome
Accession NZ_CP065441
Organism Klebsiella pneumoniae strain Patrick Star

Toxin (Protein)


Gene name hipA Uniprot ID A0A0H3GN97
Locus tag I5M63_RS02355 Protein ID WP_004199851.1
Coordinates 452525..453847 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag I5M63_RS02360 Protein ID WP_002904790.1
Coordinates 453847..454113 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M63_RS02340 447590..450091 + 2502 WP_004199853.1 fimbrial biogenesis outer membrane usher protein -
I5M63_RS02345 450079..451083 + 1005 WP_004199852.1 type 1 fimbrial protein -
I5M63_RS02350 451216..452508 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
I5M63_RS02355 452525..453847 - 1323 WP_004199851.1 type II toxin-antitoxin system HipA family toxin Toxin
I5M63_RS02360 453847..454113 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
I5M63_RS02365 454379..454705 - 327 WP_002904788.1 hydroxyisourate hydrolase -
I5M63_RS02370 454702..455202 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
I5M63_RS02375 455180..456559 - 1380 WP_004199850.1 purine permease -
I5M63_RS02380 456905..458059 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
I5M63_RS02385 458040..458963 - 924 WP_004199849.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49128.44 Da        Isoelectric Point: 7.2487

>T182660 WP_004199851.1 NZ_CP065441:c453847-452525 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T182660 NZ_CP086526:c4178913-4178740 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT182660 WP_002904790.1 NZ_CP065441:c454113-453847 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT182660 NZ_CP086526:c4179237-4178941 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GN97

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References