Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1735609..1736228 Replicon chromosome
Accession NZ_CP065438
Organism Klebsiella pneumoniae strain Mrs. Puff

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I5M55_RS08340 Protein ID WP_002892050.1
Coordinates 1735609..1735827 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I5M55_RS08345 Protein ID WP_002892066.1
Coordinates 1735854..1736228 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M55_RS08305 1731656..1731919 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
I5M55_RS08310 1731919..1732059 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I5M55_RS08315 1732056..1732754 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
I5M55_RS08320 1732855..1734306 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
I5M55_RS08325 1734281..1734751 - 471 WP_002892026.1 YlaC family protein -
I5M55_RS08330 1734772..1734912 + 141 WP_004147370.1 hypothetical protein -
I5M55_RS08335 1734884..1735450 - 567 WP_002892030.1 maltose O-acetyltransferase -
I5M55_RS08340 1735609..1735827 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I5M55_RS08345 1735854..1736228 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I5M55_RS08350 1736714..1739860 - 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
I5M55_RS08355 1739883..1741076 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182642 WP_002892050.1 NZ_CP065438:c1735827-1735609 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T182642 NZ_CP086520:3885233-3885385 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT182642 WP_002892066.1 NZ_CP065438:c1736228-1735854 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT182642 NZ_CP086520:c3885184-3885127 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References