Detailed information of TA system TA182635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 114907..115550
• Replicon: plasmid pDirtyDan_01
• Accession: NZ_CP065437
• Organism: Klebsiella pneumoniae strain Dirty Dan

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: I6Z21_RS26175
• Protein ID: WP_021312475.1
• Coordinates: 114907..115323 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6B7Q5S4
• Locus tag: I6Z21_RS26180
• Protein ID: WP_021312476.1
• Coordinates: 115320..115550 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6Z21_RS26125	110194..110289	-	96	Protein_131	IS3 family transposase	-
I6Z21_RS26130	110379..110645	-	267	Protein_132	ISNCY family transposase	-
I6Z21_RS26135	110935..111801	+	867	WP_052264179.1	hypothetical protein	-
I6Z21_RS26140	111956..112243	+	288	WP_042938777.1	chorismate mutase	-
I6Z21_RS26145	112345..112839	-	495	WP_065808010.1	DNA-binding protein	-
I6Z21_RS26150	112870..113445	-	576	WP_065808008.1	hypothetical protein	-
I6Z21_RS26155	113486..113701	-	216	WP_065808007.1	hypothetical protein	-
I6Z21_RS26160	113940..114266	-	327	WP_124989605.1	hypothetical protein	-
I6Z21_RS26165	114345..114563	-	219	WP_021312527.1	hypothetical protein	-
I6Z21_RS26170	114627..114872	-	246	WP_065905238.1	hypothetical protein	-
I6Z21_RS26175	114907..115323	-	417	WP_021312475.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6Z21_RS26180	115320..115550	-	231	WP_021312476.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6Z21_RS26185	115870..117435	+	1566	WP_094716520.1	ATP-binding protein	-
I6Z21_RS26190	117492..118361	-	870	WP_094716519.1	hypothetical protein	-
I6Z21_RS26195	118363..119451	-	1089	WP_094716522.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaDHA-1 / qnrB4 / aph(6)-Id / blaTEM-1B / aac(3)-IId / floR / sul2 / aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / tet(A)	-	1..147528	147528

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15033.44 Da
• Isoelectric Point: 7.8915

>T182635 WP_021312475.1 NZ_CP065437:c115323-114907 [Klebsiella pneumoniae]
VKKTFMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T182635 NZ_CP086520:1109303-1109614 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8723.76 Da
• Isoelectric Point: 4.3410

>AT182635 WP_021312476.1 NZ_CP065437:c115550-115320 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT182635 NZ_CP086520:1109611-1110030 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A6B7Q5S4