Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3919572..3920197 Replicon chromosome
Accession NZ_CP065436
Organism Klebsiella pneumoniae strain Dirty Dan

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I6Z21_RS19140 Protein ID WP_002882817.1
Coordinates 3919572..3919955 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I6Z21_RS19145 Protein ID WP_004150355.1
Coordinates 3919955..3920197 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6Z21_RS19125 3916938..3917840 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I6Z21_RS19130 3917837..3918472 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I6Z21_RS19135 3918469..3919398 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I6Z21_RS19140 3919572..3919955 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I6Z21_RS19145 3919955..3920197 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I6Z21_RS19150 3920402..3921319 + 918 WP_004150353.1 alpha/beta hydrolase -
I6Z21_RS19155 3921333..3922274 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I6Z21_RS19160 3922319..3922756 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6Z21_RS19165 3922753..3923613 - 861 WP_004146232.1 virulence factor BrkB family protein -
I6Z21_RS19170 3923607..3924206 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182630 WP_002882817.1 NZ_CP065436:c3919955-3919572 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182630 NZ_CP086520:337811-338338 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182630 WP_004150355.1 NZ_CP065436:c3920197-3919955 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182630 NZ_CP086520:337538-337804 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References