Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2731516..2732135 Replicon chromosome
Accession NZ_CP065436
Organism Klebsiella pneumoniae strain Dirty Dan

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I6Z21_RS13500 Protein ID WP_002892050.1
Coordinates 2731917..2732135 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I6Z21_RS13495 Protein ID WP_002892066.1
Coordinates 2731516..2731890 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6Z21_RS13485 2726668..2727861 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6Z21_RS13490 2727884..2731030 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
I6Z21_RS13495 2731516..2731890 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I6Z21_RS13500 2731917..2732135 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I6Z21_RS13505 2732294..2732860 + 567 WP_002892030.1 maltose O-acetyltransferase -
I6Z21_RS13510 2732832..2732972 - 141 WP_004147370.1 hypothetical protein -
I6Z21_RS13515 2732993..2733463 + 471 WP_002892026.1 YlaC family protein -
I6Z21_RS13520 2733438..2734889 - 1452 WP_194428768.1 PLP-dependent aminotransferase family protein -
I6Z21_RS13525 2734990..2735688 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
I6Z21_RS13530 2735685..2735825 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I6Z21_RS13535 2735825..2736088 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182626 WP_002892050.1 NZ_CP065436:2731917-2732135 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T182626 NZ_CP086515:8292-8552 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACATTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT182626 WP_002892066.1 NZ_CP065436:2731516-2731890 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT182626 NZ_CP086515:8542-8793 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References