Detailed information of TA system TA182595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 3965120..3965756
• Replicon: chromosome
• Accession: NZ_CP065425
• Organism: Bacillus vini strain JCM 19841

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0Q3WTD1
• Locus tag: I5776_RS19705
• Protein ID: WP_055741345.1
• Coordinates: 3965120..3965470 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: I5776_RS19710
• Protein ID: WP_202778191.1
• Coordinates: 3965475..3965756 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5776_RS19670	3960654..3961451	-	798	WP_202778184.1	RNA polymerase sigma factor SigB	-
I5776_RS19675	3961429..3961902	-	474	WP_202778185.1	anti-sigma B factor RsbW	-
I5776_RS19680	3961902..3962231	-	330	WP_202778186.1	anti-sigma factor antagonist	-
I5776_RS19685	3962298..3963305	-	1008	WP_202778187.1	PP2C family protein-serine/threonine phosphatase	-
I5776_RS19690	3963318..3963719	-	402	WP_202778188.1	anti-sigma regulatory factor	-
I5776_RS19695	3963724..3964080	-	357	WP_202778189.1	STAS domain-containing protein	-
I5776_RS19700	3964077..3964910	-	834	WP_202778190.1	STAS domain-containing protein	-
I5776_RS19705	3965120..3965470	-	351	WP_055741345.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
I5776_RS19710	3965475..3965756	-	282	WP_202778191.1	antitoxin endoai	Antitoxin
I5776_RS19715	3965930..3967087	-	1158	WP_202778192.1	alanine racemase	-
I5776_RS19720	3967231..3968253	-	1023	WP_202778193.1	outer membrane lipoprotein carrier protein LolA	-
I5776_RS19725	3968462..3968815	-	354	WP_202778194.1	holo-ACP synthase	-
I5776_RS19730	3968890..3969492	+	603	WP_202778195.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13020.06 Da
• Isoelectric Point: 4.8998

>T182595 WP_055741345.1 NZ_CP065425:c3965470-3965120 [Bacillus vini]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEEMMEKVDDALQISLGLIEF

Nucleotide

• Length: 351 bp

>T182595 NZ_CP086505:8292-8552 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACATTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10852.39 Da
• Isoelectric Point: 5.9081

>AT182595 WP_202778191.1 NZ_CP065425:c3965756-3965475 [Bacillus vini]
VSESSATTEILIRLPQQLLIELDGFAEQENMNRNEFIYRATKMYLRERKKRQIRESMRRGYMEMAKINLAIASEAIQAEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT182595 NZ_CP086505:8542-8793 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A0Q3WTD1