Detailed information of TA system TA182594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4824045..4824681
• Replicon: chromosome
• Accession: NZ_CP065424
• Organism: Heyndrickxia oleronia strain DSM 9356

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0Q3WTD1
• Locus tag: I5818_RS24195
• Protein ID: WP_055741345.1
• Coordinates: 4824045..4824395 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A8E2I5A7
• Locus tag: I5818_RS24200
• Protein ID: WP_058005316.1
• Coordinates: 4824400..4824681 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5818_RS24160 (I5818_24175)	4819253..4820050	-	798	WP_078109473.1	RNA polymerase sigma factor SigB	-
I5818_RS24165 (I5818_24180)	4820028..4820501	-	474	WP_058005322.1	anti-sigma B factor RsbW	-
I5818_RS24170 (I5818_24185)	4820501..4820830	-	330	WP_058005321.1	anti-sigma factor antagonist	-
I5818_RS24175 (I5818_24190)	4820906..4821913	-	1008	WP_078109472.1	PP2C family protein-serine/threonine phosphatase	-
I5818_RS24180 (I5818_24195)	4821925..4822326	-	402	WP_058005319.1	anti-sigma regulatory factor	-
I5818_RS24185 (I5818_24200)	4822331..4822693	-	363	WP_139254832.1	STAS domain-containing protein	-
I5818_RS24190 (I5818_24205)	4822684..4823514	-	831	WP_058005317.1	RsbT co-antagonist protein RsbRA	-
I5818_RS24195 (I5818_24210)	4824045..4824395	-	351	WP_055741345.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
I5818_RS24200 (I5818_24215)	4824400..4824681	-	282	WP_058005316.1	hypothetical protein	Antitoxin
I5818_RS24205 (I5818_24220)	4824851..4826026	-	1176	WP_078111168.1	alanine racemase	-
I5818_RS24210 (I5818_24225)	4826386..4827402	-	1017	WP_058005315.1	outer membrane lipoprotein carrier protein LolA	-
I5818_RS24215 (I5818_24230)	4827607..4827960	-	354	WP_058005314.1	holo-ACP synthase	-
I5818_RS24220 (I5818_24235)	4828038..4828628	+	591	WP_078111169.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13020.06 Da
• Isoelectric Point: 4.8998

>T182594 WP_055741345.1 NZ_CP065424:c4824395-4824045 [Heyndrickxia oleronia]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEEMMEKVDDALQISLGLIEF

Nucleotide

• Length: 351 bp

>T182594 NZ_CP086503:41470-41850 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACAGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10774.26 Da
• Isoelectric Point: 6.4777

>AT182594 WP_058005316.1 NZ_CP065424:c4824681-4824400 [Heyndrickxia oleronia]
VSESSATTEILIRLPVHLLSELDGFAEQENVNRNEFIYRATKMYLRERKKRQIRESMRRGYMEMAKINLAIASEAIQAEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT182594 NZ_CP086503:41249-41470 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGAGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A0Q3WTD1