Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 5652588..5653174 Replicon chromosome
Accession NZ_CP065417
Organism Pseudomonas aeruginosa isolate P23

Toxin (Protein)


Gene name PumA Uniprot ID G8CP73
Locus tag I5U72_RS26235 Protein ID WP_003120987.1
Coordinates 5652875..5653174 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID -
Locus tag I5U72_RS26230 Protein ID WP_003448662.1
Coordinates 5652588..5652878 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5U72_RS26215 5648170..5650059 + 1890 WP_196994395.1 hypothetical protein -
I5U72_RS26220 5650056..5652032 + 1977 WP_016851611.1 DEAD/DEAH box helicase -
I5U72_RS26225 5652173..5652517 + 345 WP_016851612.1 hypothetical protein -
I5U72_RS26230 5652588..5652878 - 291 WP_003448662.1 putative addiction module antidote protein Antitoxin
I5U72_RS26235 5652875..5653174 - 300 WP_003120987.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I5U72_RS26240 5653376..5654500 + 1125 WP_012076859.1 TcpQ domain-containing protein -
I5U72_RS26245 5654500..5656209 + 1710 WP_003099757.1 PilN family type IVB pilus formation outer membrane protein -
I5U72_RS26250 5656213..5657538 + 1326 WP_003099758.1 type 4b pilus protein PilO2 -
I5U72_RS26255 5657528..5658061 + 534 WP_003099760.1 type IV pilus biogenesis protein PilP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 5624559..5711771 87212


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(47-90)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11133.88 Da        Isoelectric Point: 10.4495

>T182589 WP_003120987.1 NZ_CP065417:c5653174-5652875 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Download         Length: 300 bp

>T182589 NZ_CP086501:69724-69876 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10394.91 Da        Isoelectric Point: 4.7527

>AT182589 WP_003448662.1 NZ_CP065417:c5652878-5652588 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Download         Length: 291 bp

>AT182589 NZ_CP086501:c69674-69612 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G8CP73

Antitoxin

Source ID Structure

References