Detailed information of TA system TA182587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4720154..4721412
• Replicon: chromosome
• Accession: NZ_CP065417
• Organism: Pseudomonas aeruginosa isolate P23

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: I5U72_RS21830
• Protein ID: WP_003112528.1
• Coordinates: 4720154..4720909 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: I5U72_RS21835
• Protein ID: WP_003086329.1
• Coordinates: 4721044..4721412 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5U72_RS21820	4715327..4717870	+	2544	WP_031755663.1	acyl-homoserine-lactone acylase QuiP	-
I5U72_RS21825	4718025..4719386	-	1362	WP_073647918.1	DNA recombination protein RmuC	-
I5U72_RS21830	4720154..4720909	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
I5U72_RS21835	4721044..4721412	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
I5U72_RS21840	4721485..4722771	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
I5U72_RS21845	4722827..4724416	-	1590	WP_074231416.1	aldehyde dehydrogenase family protein	-
I5U72_RS21850	4724495..4724971	+	477	WP_003112531.1	hypothetical protein	-
I5U72_RS21855	4724974..4725249	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T182587 WP_003112528.1 NZ_CP065417:c4720909-4720154 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T182587 NZ_CP086500:c4346287-4346114 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT182587 WP_003086329.1 NZ_CP065417:c4721412-4721044 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT182587 NZ_CP086500:c4346611-4346315 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7