Detailed information of TA system TA182584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1637143..1638838
• Replicon: chromosome
• Accession: NZ_CP065417
• Organism: Pseudomonas aeruginosa isolate P23

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: I5U72_RS07670
• Protein ID: WP_003112901.1
• Coordinates: 1637143..1638012 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: I5U72_RS07675
• Protein ID: WP_003112902.1
• Coordinates: 1638005..1638838 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5U72_RS07645	1633151..1634002	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
I5U72_RS07650	1634043..1635050	+	1008	WP_003162724.1	ABC transporter substrate-binding protein	-
I5U72_RS07655	1635047..1635823	+	777	WP_003112898.1	ABC transporter permease	-
I5U72_RS07660	1635829..1636590	+	762	WP_003112899.1	SDR family oxidoreductase	-
I5U72_RS07665	1636604..1637134	+	531	WP_003112900.1	cupin domain-containing protein	-
I5U72_RS07670	1637143..1638012	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
I5U72_RS07675	1638005..1638838	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
I5U72_RS07680	1638825..1639622	+	798	WP_017001845.1	SDR family oxidoreductase	-
I5U72_RS07685	1639615..1641297	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
I5U72_RS07690	1641308..1642111	+	804	WP_003453564.1	aspartate dehydrogenase	-
I5U72_RS07695	1642123..1643607	+	1485	WP_031756606.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T182584 WP_003112901.1 NZ_CP065417:1637143-1638012 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T182584 NZ_CP086500:3792684-3792962 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT182584 WP_003112902.1 NZ_CP065417:1638005-1638838 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT182584 NZ_CP086500:3792421-3792681 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1