Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4205320..4205936 Replicon chromosome
Accession NZ_CP065411
Organism Kosakonia radicincitans strain IAC-BECa95

Toxin (Protein)


Gene name Hha Uniprot ID A0A1C4FBY4
Locus tag I5960_RS20380 Protein ID WP_007373583.1
Coordinates 4205718..4205936 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A6M5CWA9
Locus tag I5960_RS20375 Protein ID WP_007373584.1
Coordinates 4205320..4205694 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5960_RS20365 4200441..4201634 + 1194 WP_007373586.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I5960_RS20370 4201657..4204806 + 3150 WP_007373585.1 multidrug efflux RND transporter permease subunit AcrB -
I5960_RS20375 4205320..4205694 + 375 WP_007373584.1 Hha toxicity modulator TomB Antitoxin
I5960_RS20380 4205718..4205936 + 219 WP_007373583.1 HHA domain-containing protein Toxin
I5960_RS20385 4206002..4206562 + 561 WP_007373582.1 maltose O-acetyltransferase -
I5960_RS20390 4206663..4207142 + 480 WP_035887316.1 YlaC family protein -
I5960_RS20395 4207108..4208553 - 1446 WP_161518096.1 PLP-dependent aminotransferase family protein -
I5960_RS20400 4208651..4209352 + 702 WP_035887312.1 GNAT family protein -
I5960_RS20405 4209349..4209489 - 141 WP_007373577.1 type B 50S ribosomal protein L36 -
I5960_RS20410 4209493..4209753 - 261 WP_007373576.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8637.05 Da        Isoelectric Point: 7.9893

>T182579 WP_007373583.1 NZ_CP065411:4205718-4205936 [Kosakonia radicincitans]
MSEKPLTKVDYLMRLRRCQTIDTLERVIEKNKYELPDEELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T182579 NZ_CP086500:c2542207-2542031 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14567.42 Da        Isoelectric Point: 5.8339

>AT182579 WP_007373584.1 NZ_CP065411:4205320-4205694 [Kosakonia radicincitans]
MDEYSPKRHDIAQLKFLCETLYHDCLVNLEESHHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTHDLQKWRKTGNKLFRCFVNVSKANPVSHSC

Download         Length: 375 bp

>AT182579 NZ_CP086500:c2541985-2541569 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C4FBY4

Antitoxin

Source ID Structure
AlphaFold DB A0A6M5CWA9

References