Detailed information of TA system TA182576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 3942600..3943394
• Replicon: chromosome
• Accession: NZ_CP065411
• Organism: Kosakonia radicincitans strain IAC-BECa95

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: I5960_RS19180
• Protein ID: WP_227135851.1
• Coordinates: 3942873..3943394 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: I5960_RS19175
• Protein ID: WP_007373817.1
• Coordinates: 3942600..3942869 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5960_RS19150	3937929..3938618	+	690	WP_227135844.1	acireductone synthase	-
I5960_RS19155	3938615..3939157	+	543	WP_227135846.1	acireductone dioxygenase	-
I5960_RS19160	3939559..3940671	+	1113	WP_227135847.1	AAA family ATPase	-
I5960_RS19165	3940676..3941557	+	882	WP_227135849.1	SIR2 family protein	-
I5960_RS19170	3942077..3942394	+	318	WP_064567913.1	hypothetical protein	-
I5960_RS19175	3942600..3942869	+	270	WP_007373817.1	DUF1778 domain-containing protein	Antitoxin
I5960_RS19180	3942873..3943394	+	522	WP_227135851.1	GNAT family N-acetyltransferase	Toxin
I5960_RS19185	3943592..3944164	+	573	WP_227135853.1	DUF1349 domain-containing protein	-
I5960_RS19190	3944595..3945356	+	762	WP_227135854.1	MCP four helix bundle domain-containing protein	-
I5960_RS19195	3945356..3946132	+	777	WP_227135855.1	methyl-accepting chemotaxis protein	-
I5960_RS19200	3946217..3946480	-	264	WP_007373812.1	MafI family immunity protein	-
I5960_RS19205	3946477..3946806	-	330	WP_071920469.1	SdpI family protein	-
I5960_RS19210	3947036..3947809	-	774	WP_007373810.1	hypothetical protein	-
I5960_RS19215	3947812..3948009	-	198	WP_007373809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19419.32 Da
• Isoelectric Point: 8.4536

>T182576 WP_227135851.1 NZ_CP065411:3942873-3943394 [Kosakonia radicincitans]
VDNLTIEMFSGETEYETGGFDCGEVSLNAFLTNHLKRQHDGKILRAWLLVTKTPRPRILGYYTLSGGSFEKEQLPTKTQQ
KKVPYRNVPCVILGRLAIDKSLQGNGWGEILVAHAMRVVYTASLNIGVHGLFVEALTGNAKKFYEKLGFIPLADGNQHSL
FYPTKSIERLFEE

Nucleotide

• Length: 522 bp

>T182576 NZ_CP086500:c1920891-1920514 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10273.77 Da
• Isoelectric Point: 7.0179

>AT182576 WP_007373817.1 NZ_CP065411:3942600-3942869 [Kosakonia radicincitans]
MPALKKQRIDLRLTDEDKKMIEEAAAMTNQTVTQFLVNSASERAAEVIEQHRRLILSEESWNRVMDAIENPPAPNDKLKR
AAKRLQNME

Nucleotide

• Length: 270 bp

>AT182576 NZ_CP086500:c1921349-1920981 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCACCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA