Detailed information of TA system TA182565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1663543..1664176
• Replicon: chromosome
• Accession: NZ_CP065411
• Organism: Kosakonia radicincitans strain IAC-BECa95

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: I5960_RS08280
• Protein ID: WP_227136565.1
• Coordinates: 1663543..1663719 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I5960_RS08285
• Protein ID: WP_227136566.1
• Coordinates: 1663769..1664176 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5960_RS08255	1659098..1659292	+	195	WP_227136560.1	Cro/CI family transcriptional regulator	-
I5960_RS08260	1659304..1659591	+	288	WP_227136561.1	hypothetical protein	-
I5960_RS08265	1659912..1661522	+	1611	WP_227136562.1	DEAD/DEAH box helicase	-
I5960_RS08270	1661519..1662493	+	975	WP_227136563.1	toprim domain-containing protein	-
I5960_RS08275	1662490..1663305	+	816	WP_227136564.1	antitermination protein	-
I5960_RS08280	1663543..1663719	+	177	WP_227136565.1	type II toxin-antitoxin system HicA family toxin	Toxin
I5960_RS08285	1663769..1664176	+	408	WP_227136566.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I5960_RS08290	1664325..1664609	+	285	WP_107146513.1	phage holin	-
I5960_RS08295	1664612..1665142	+	531	WP_227136567.1	lysozyme	-
I5960_RS08300	1665139..1665672	+	534	WP_227136568.1	hypothetical protein	-
I5960_RS08305	1665675..1665899	+	225	WP_227136569.1	hypothetical protein	-
I5960_RS08310	1666001..1666261	+	261	WP_227136844.1	hypothetical protein	-
I5960_RS08315	1666289..1666549	+	261	WP_227136570.1	DUF1378 family protein	-
I5960_RS08320	1666698..1667117	+	420	WP_227136571.1	hypothetical protein	-
I5960_RS08325	1667121..1667372	+	252	WP_227136572.1	hypothetical protein	-
I5960_RS08330	1667451..1667951	+	501	WP_144136158.1	prophage tail fiber N-terminal domain-containing protein	-
I5960_RS08335	1667951..1668262	+	312	WP_227136573.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1653224..1694554	41330

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-62)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6626.82 Da
• Isoelectric Point: 11.4111

>T182565 WP_227136565.1 NZ_CP065411:1663543-1663719 [Kosakonia radicincitans]
VKQSEFRRWLVAQGVEVKDGTNHLKLSLNGKQTVMPRHPGKEIPEPLRKAILRQLGLK

Nucleotide

• Length: 177 bp

>T182565 NZ_CP086495:8292-8552 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACATTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14700.71 Da
• Isoelectric Point: 4.3472

>AT182565 WP_227136566.1 NZ_CP065411:1663769-1664176 [Kosakonia radicincitans]
MRYPVTFDCDDTGCAVLFPDIPEAMTGGDTREEALALAQDALVTAFDFYFEDRREIPQPSKEGEAFVEVPASVAAKVLLL
NAVVQSGVSNAELARMIDTRPQEVTRILDLHHSTKIDTIQKALAALGKTLELHAA

Nucleotide

• Length: 408 bp

>AT182565 NZ_CP086495:8542-8793 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA