Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 280549..281192 Replicon chromosome
Accession NZ_CP065410
Organism Pseudomonas sp. IAC-BECa141

Toxin (Protein)


Gene name hicA Uniprot ID A0A345RIP1
Locus tag I5961_RS01225 Protein ID WP_065261145.1
Coordinates 280549..280731 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag I5961_RS01230 Protein ID WP_085684410.1
Coordinates 280791..281192 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5961_RS01205 (I5961_01205) 275624..276628 - 1005 WP_085702744.1 D-cysteine desulfhydrase -
I5961_RS01210 (I5961_01210) 276819..277745 + 927 WP_227234114.1 serine acetyltransferase -
I5961_RS01215 (I5961_01215) 278034..279995 + 1962 WP_176247724.1 choline transporter BetT -
I5961_RS01220 (I5961_01220) 280020..280280 - 261 WP_085697997.1 hypothetical protein -
I5961_RS01225 (I5961_01225) 280549..280731 + 183 WP_065261145.1 type II toxin-antitoxin system HicA family toxin Toxin
I5961_RS01230 (I5961_01230) 280791..281192 + 402 WP_085684410.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I5961_RS01235 (I5961_01235) 281215..281952 - 738 Protein_239 substrate-binding domain-containing protein -
I5961_RS01240 (I5961_01240) 281956..283050 + 1095 Protein_240 TonB-dependent receptor -
I5961_RS01245 (I5961_01245) 283052..284371 + 1320 WP_227234115.1 LLM class flavin-dependent oxidoreductase -
I5961_RS01250 (I5961_01250) 284411..285448 + 1038 WP_085702740.1 L-glyceraldehyde 3-phosphate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6836.96 Da        Isoelectric Point: 10.5417

>T182559 WP_065261145.1 NZ_CP065410:280549-280731 [Pseudomonas sp. IAC-BECa141]
MRSREMIRMIEEDGWYLVAVKGSHHQYKHPGKPGRVTIKHPDSDLPKGTINSILKQAGLK

Download         Length: 183 bp

>T182559 NZ_CP086491:68036-68188 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14555.29 Da        Isoelectric Point: 4.3652

>AT182559 WP_085684410.1 NZ_CP065410:280791-281192 [Pseudomonas sp. IAC-BECa141]
MKFPVVLHKDADSDYGVIIPDVPGCFSAGTTVAEAFDNTQEALALHYEGLVADGAPLPRVQDIDAHLENPDYAGGIWGVV
EFDITPYFGKSVRFNATLPEQLLERIDQTVRRDQRYSSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT182559 NZ_CP086491:c67986-67924 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A345RIP1

Antitoxin

Source ID Structure

References