Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 36106..36637 Replicon plasmid pDCB2
Accession NZ_CP065408
Organism Diaphorobacter sp. JS3051

Toxin (Protein)


Gene name relE Uniprot ID I2ALI9
Locus tag I3K84_RS21465 Protein ID WP_011114071.1
Coordinates 36356..36637 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID I2ALI8
Locus tag I3K84_RS21460 Protein ID WP_011114070.1
Coordinates 36106..36369 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3K84_RS21415 31312..32076 - 765 WP_011114061.1 ParA family protein -
I3K84_RS21420 32073..32381 - 309 WP_011114062.1 KorA protein -
I3K84_RS21425 32488..32817 - 330 WP_011114064.1 DUF2761 domain-containing protein -
I3K84_RS21430 32819..33145 - 327 WP_011114065.1 hypothetical protein -
I3K84_RS21435 33354..33590 - 237 WP_011114066.1 stable inheritance protein KleA -
I3K84_RS21440 33748..34005 - 258 WP_011114067.1 hypothetical protein -
I3K84_RS21445 34022..35230 - 1209 WP_011114068.1 hypothetical protein -
I3K84_RS21450 35242..35505 - 264 WP_011296181.1 hypothetical protein -
I3K84_RS21455 35505..35933 - 429 WP_011114069.1 antirestriction protein -
I3K84_RS21460 36106..36369 + 264 WP_011114070.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
I3K84_RS21465 36356..36637 + 282 WP_011114071.1 type II toxin-antitoxin system YafQ family toxin Toxin
I3K84_RS21470 37907..40609 - 2703 WP_196996221.1 Tn3 family transposase -
I3K84_RS21475 40755..40940 + 186 Protein_42 nucleoside deaminase -
I3K84_RS21480 40959..41213 - 255 WP_049756531.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..77623 77623
- flank IS/Tn - - 37907..40609 2702


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-83)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10743.19 Da        Isoelectric Point: 7.4750

>T182558 WP_011114071.1 NZ_CP065408:36356-36637 [Diaphorobacter sp. JS3051]
MRQIERTGQFKRDYKREAKGQHRATLDADLVPVLVALADDQPLEPRHRDHALTGDWKDHRDCHVKPDLVLIYQKPDADTL
RLVRLGSHSELGL

Download         Length: 282 bp

>T182558 NZ_CP086491:2255-2533 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9255.82 Da        Isoelectric Point: 5.7905

>AT182558 WP_011114070.1 NZ_CP065408:36106-36369 [Diaphorobacter sp. JS3051]
MAANQLVQTRIDGAIKEEAAAVLAAMGLTVSDAVRLLLTKVAQDKALPFEPLIPNATTIEAMKEARKGKLPRFATVNDLM
ADLHAAD

Download         Length: 264 bp

>AT182558 NZ_CP086491:1986-2255 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1U9V4I5

Antitoxin

Source ID Structure
AlphaFold DB A0A1U9V3I2

References