Detailed information of TA system TA182557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 87496..88174
• Replicon: plasmid pDCB1
• Accession: NZ_CP065407
• Organism: Diaphorobacter sp. JS3051

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: I3K84_RS21175
• Protein ID: WP_196996168.1
• Coordinates: 87818..88174 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: I3K84_RS21170
• Protein ID: WP_196996167.1
• Coordinates: 87496..87816 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K84_RS21140	82982..83359	-	378	WP_196996163.1	hypothetical protein	-
I3K84_RS21145	83370..83642	-	273	WP_196996164.1	hypothetical protein	-
I3K84_RS21150	84038..84859	-	822	WP_008903250.1	IS21-like element helper ATPase IstB	-
I3K84_RS21155	84849..86381	-	1533	WP_011804189.1	IS21-like element ISAisp6 family transposase	-
I3K84_RS21160	86476..86718	-	243	WP_196996165.1	DUF2281 domain-containing protein	-
I3K84_RS21165	86843..87361	+	519	WP_196996166.1	transcription/translation regulatory transformer protein RfaH	-
I3K84_RS21170	87496..87816	-	321	WP_196996167.1	helix-turn-helix domain-containing protein	Antitoxin
I3K84_RS21175	87818..88174	-	357	WP_196996168.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3K84_RS21180	88467..89657	-	1191	WP_196996169.1	glycosyltransferase family 4 protein	-
I3K84_RS21185	89668..90804	-	1137	WP_196996170.1	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)	-
I3K84_RS21190	90813..91940	-	1128	WP_196996171.1	capsular polysaccharide biosynthesis protein CapF	-
I3K84_RS21195	91945..92991	-	1047	WP_196996172.1	polysaccharide biosynthesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cap8E / bplB	1..108781	108781

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-86)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13036.05 Da
• Isoelectric Point: 8.1065

>T182557 WP_196996168.1 NZ_CP065407:c88174-87818 [Diaphorobacter sp. JS3051]
MPDHIKQLQWVGSAKKDLLAMPEDVIDVFGFALHLAQTGKKHANAKPLRGFGGAGVLEVVEDHLGDTYRAVYTVKIDEAV
YVLHCFQKKSKQGIETPKHDMDLIRERLKAAQADAKGA

Nucleotide

• Length: 357 bp

>T182557 NZ_CP086490:c4346289-4346116 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11462.39 Da
• Isoelectric Point: 9.8341

>AT182557 WP_196996167.1 NZ_CP065407:c87816-87496 [Diaphorobacter sp. JS3051]
MIEIEKGSANVYEDLGLPDASEMLVKATLAAKIGEIIKHRHLTQVQASEILGMPQPKLSGMLRGQFRGISEAKMLECLNR
LGRNVEIVVSKPSRSGSAGRTSVVFA

Nucleotide

• Length: 321 bp

>AT182557 NZ_CP086490:c4346613-4346317 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG