Detailed information of TA system TA182556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 74079..74772
• Replicon: plasmid pDCB1
• Accession: NZ_CP065407
• Organism: Diaphorobacter sp. JS3051

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: I3K84_RS21095
• Protein ID: WP_196996158.1
• Coordinates: 74395..74772 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: I3K84_RS21090
• Protein ID: WP_196996157.1
• Coordinates: 74079..74414 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K84_RS21065	69880..72459	+	2580	WP_196996153.1	EAL domain-containing protein	-
I3K84_RS21070	72604..72966	-	363	Protein_88	hypothetical protein	-
I3K84_RS21075	72963..73289	-	327	WP_196996154.1	hypothetical protein	-
I3K84_RS21080	73313..73708	-	396	WP_196996155.1	hypothetical protein	-
I3K84_RS21085	73705..74064	-	360	WP_196996156.1	transposase	-
I3K84_RS21090	74079..74414	-	336	WP_196996157.1	helix-turn-helix domain-containing protein	Antitoxin
I3K84_RS21095	74395..74772	-	378	WP_196996158.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3K84_RS21100	74964..75554	+	591	WP_196996159.1	recombinase family protein	-
I3K84_RS21105	75559..78519	+	2961	WP_196996160.1	Tn3 family transposase	-
I3K84_RS21110	78539..78772	-	234	Protein_96	type II toxin-antitoxin system RelE/ParE family toxin	-
I3K84_RS21115	78840..79220	-	381	WP_004637276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cap8E / bplB	1..108781	108781
	-	flank	IS/Tn	-	-	74964..75554	590

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13774.90 Da
• Isoelectric Point: 8.8594

>T182556 WP_196996158.1 NZ_CP065407:c74772-74395 [Diaphorobacter sp. JS3051]
MTDKEKPLEWIASSYKDLMALPPDVRRRFGYALSLAQMGDQDDAAKVLKGFGGAGVLEVIEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKVDMDIIRARLKVAEALAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T182556 NZ_CP086490:c4244120-4243770 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12156.30 Da
• Isoelectric Point: 10.3419

>AT182556 WP_196996157.1 NZ_CP065407:c74414-74079 [Diaphorobacter sp. JS3051]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRKAMRALGLTQQAAAKRMGIPQPKVSGMMHGDFTNLSERK
LMDCLNRLGYDIEIKVRPASEPVGHLTLATA

Nucleotide

• Length: 336 bp

>AT182556 NZ_CP086490:c4244365-4244114 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA