Detailed information of TA system TA182554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3964747..3965789
• Replicon: chromosome
• Accession: NZ_CP065406
• Organism: Diaphorobacter sp. JS3051

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: I3K84_RS18865
• Protein ID: WP_003153636.1
• Coordinates: 3965214..3965789 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: I3K84_RS18860
• Protein ID: WP_003050245.1
• Coordinates: 3964747..3965217 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K84_RS18825	3960130..3961560	-	1431	WP_011804670.1	TIGR03752 family integrating conjugative element protein	-
I3K84_RS18830	3961550..3962458	-	909	WP_009516219.1	TIGR03749 family integrating conjugative element protein	-
I3K84_RS18835	3962455..3963147	-	693	WP_009516220.1	TIGR03746 family integrating conjugative element protein	-
I3K84_RS18840	3963144..3963542	-	399	WP_009516221.1	TIGR03750 family conjugal transfer protein	-
I3K84_RS18845	3963554..3963913	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
I3K84_RS18850	3963930..3964163	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
I3K84_RS18855	3964160..3964543	-	384	WP_011804671.1	RAQPRD family integrative conjugative element protein	-
I3K84_RS18860	3964747..3965217	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
I3K84_RS18865	3965214..3965789	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
I3K84_RS18870	3965807..3966721	+	915	WP_003050256.1	AAA family ATPase	-
I3K84_RS18875	3966718..3967188	+	471	WP_011804672.1	hypothetical protein	-
I3K84_RS18880	3967185..3967685	+	501	WP_011804673.1	hypothetical protein	-
I3K84_RS18885	3967685..3968587	+	903	WP_011804674.1	nucleotidyltransferase	-
I3K84_RS18890	3968626..3969351	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3923017..3985972	62955

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T182554 WP_003153636.1 NZ_CP065406:3965214-3965789 [Diaphorobacter sp. JS3051]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T182554 NZ_CP086490:3792688-3792966 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT182554 WP_003050245.1 NZ_CP065406:3964747-3965217 [Diaphorobacter sp. JS3051]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT182554 NZ_CP086490:3792425-3792685 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: I3TV68