Detailed information of TA system TA182552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 3585543..3587041
• Replicon: chromosome
• Accession: NZ_CP065406
• Organism: Diaphorobacter sp. JS3051

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A7T1GP45
• Locus tag: I3K84_RS17015
• Protein ID: WP_011804354.1
• Coordinates: 3585543..3586445 (-)
• Length: 301 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: A0A7T1GP65
• Locus tag: I3K84_RS17020
• Protein ID: WP_049756454.1
• Coordinates: 3586442..3587041 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K84_RS16995	3581552..3582379	-	828	WP_196995040.1	hypothetical protein	-
I3K84_RS17000	3582379..3582975	-	597	WP_196995041.1	hypothetical protein	-
I3K84_RS17005	3582995..3584229	+	1235	WP_085947515.1	IS3 family transposase	-
I3K84_RS17010	3584316..3585530	+	1215	WP_196995042.1	hypothetical protein	-
I3K84_RS17015	3585543..3586445	-	903	WP_011804354.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
I3K84_RS17020	3586442..3587041	-	600	WP_049756454.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
I3K84_RS17025	3587114..3588463	-	1350	WP_196995043.1	site-specific integrase	-
I3K84_RS17030	3588556..3589635	-	1080	WP_005801445.1	IS630 family transposase	-
I3K84_RS17035	3589856..3590818	+	963	WP_196995044.1	IS5 family transposase	-
I3K84_RS17040	3591183..3591686	-	504	WP_196995045.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3582995..3593815	10820

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-257)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (7-52)

Sequences

Toxin

Protein

• Length: 301 a.a.
• Molecular weight: 32799.50 Da
• Isoelectric Point: 5.8761

>T182552 WP_011804354.1 NZ_CP065406:c3586445-3585543 [Diaphorobacter sp. JS3051]
MTGNLSASILARLLTLAKQRGDDYNLLLNRYGMERLLARVSTSPHADRFLLKGALLFALWYDTPHRPTRDADLLGFGPDD
EANLIATFRDIAAMDLGDGIVFDPESVKADAIREDNTYGGTRITLVARIGSARCALQIDVGFGDAVTPGPQTVAYPTLLG
DFPAPTLRVYPVYTVIAEKYQAMVMLGQANSRMKDFFDLAVIARRTELDGATLAAAIAATFARRQTALPTGRPLALTQQF
SEDAAKLRQWQAFLNKNRIEAASLGETVALLDDLLWPPTQVATASSQATATWRPEALRWV

Nucleotide

• Length: 903 bp

>T182552 NZ_CP086490:3573997-3574215 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22482.01 Da
• Isoelectric Point: 9.9492

>AT182552 WP_049756454.1 NZ_CP065406:c3587041-3586442 [Diaphorobacter sp. JS3051]
MNQSDHILELARHRRVLRAADVREHGWSPQLLIRLHQAGKLQRLGRGLYGLPDAEVTEHQTLIEVCQRVPKAVLCLLSAL
QFHEIGTQLPHEVWIALPEATQTPALSYPTLRITRLRGTAYSNGIQTVTEHGAPIRVYSAAKTVADCFKFRNKIGLDVAL
EALKDAWRSRKVTIAELSHFAKINRVDRVMQPYLEAVTQ

Nucleotide

• Length: 600 bp

>AT182552 NZ_CP086490:3573597-3573971 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7T1GP45

Antitoxin

• AlphaFold DB: A0A7T1GP65