Detailed information of TA system TA182546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 172004..172755
• Replicon: plasmid pn-1
• Accession: NZ_CP065402
• Organism: Klebsiella pneumoniae strain KPNSP-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: I5G01_RS26700
• Protein ID: WP_004902249.1
• Coordinates: 172004..172486 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: I5G01_RS26705
• Protein ID: WP_004902250.1
• Coordinates: 172477..172755 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5G01_RS26670	167010..167978	+	969	WP_004225014.1	IS5 family transposase	-
I5G01_RS26675	168035..168271	+	237	Protein_175	kinase	-
I5G01_RS26680	168403..169050	-	648	WP_014386537.1	EcsC family protein	-
I5G01_RS26685	169077..169832	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
I5G01_RS26690	169933..170325	-	393	WP_045145491.1	hypothetical protein	-
I5G01_RS26695	170430..170969	-	540	WP_004902239.1	hypothetical protein	-
I5G01_RS26700	172004..172486	-	483	WP_004902249.1	GNAT family N-acetyltransferase	Toxin
I5G01_RS26705	172477..172755	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
I5G01_RS26710	172874..173086	-	213	WP_004902255.1	hypothetical protein	-
I5G01_RS26715	173194..173535	-	342	WP_004902257.1	hypothetical protein	-
I5G01_RS26720	173996..174265	-	270	WP_071591895.1	hypothetical protein	-
I5G01_RS26725	174503..176173	-	1671	WP_004902261.1	AMP-binding protein	-
I5G01_RS26730	176154..177419	-	1266	WP_004210292.1	MSMEG_0569 family flavin-dependent oxidoreductase	-
I5G01_RS26735	177437..177727	-	291	WP_011154627.1	MSMEG_0570 family nitrogen starvation response protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..204701	204701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17689.52 Da
• Isoelectric Point: 9.2033

>T182546 WP_004902249.1 NZ_CP065402:c172486-172004 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNKVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T182546 NZ_CP086490:c1920889-1920512 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT182546 WP_004902250.1 NZ_CP065402:c172755-172477 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT182546 NZ_CP086490:c1921347-1920979 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCACCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3