Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 126853..127589 Replicon plasmid pn-1
Accession NZ_CP065402
Organism Klebsiella pneumoniae strain KPNSP-1

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag I5G01_RS26460 Protein ID WP_004098919.1
Coordinates 127107..127589 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag I5G01_RS26455 Protein ID WP_004213599.1
Coordinates 126853..127119 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5G01_RS26440 122794..123351 + 558 WP_004213590.1 recombinase family protein -
I5G01_RS26445 123354..126323 + 2970 WP_004213592.1 Tn3 family transposase -
I5G01_RS26450 126365..126679 + 315 Protein_130 DNA-binding protein -
I5G01_RS26455 126853..127119 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
I5G01_RS26460 127107..127589 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
I5G01_RS26465 128010..129237 + 1228 Protein_133 IS3 family transposase -
I5G01_RS26470 129233..129628 - 396 Protein_134 DDE-type integrase/transposase/recombinase -
I5G01_RS26475 129803..130825 - 1023 WP_004214536.1 porphobilinogen synthase -
I5G01_RS26480 130889..132235 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / rmpA2 / iutA / iucD / iucC / iucB / iucA 1..204701 204701
- inside IScluster/Tn - - 122794..129237 6443


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T182545 WP_004098919.1 NZ_CP065402:127107-127589 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T182545 NZ_CP086490:1907829-1908083 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT182545 WP_004213599.1 NZ_CP065402:126853-127119 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT182545 NZ_CP086490:1907581-1907832 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References