Detailed information of TA system TA182544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 62368..63038
• Replicon: plasmid pn-1
• Accession: NZ_CP065402
• Organism: Klebsiella pneumoniae strain KPNSP-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: I5G01_RS26135
• Protein ID: WP_004213072.1
• Coordinates: 62595..63038 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: I5G01_RS26130
• Protein ID: WP_004213073.1
• Coordinates: 62368..62598 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5G01_RS26105	58591..59490	-	900	WP_004225022.1	nucleotide-binding protein	-
I5G01_RS26110	59480..59770	-	291	WP_004213078.1	hypothetical protein	-
I5G01_RS26115	60122..60328	+	207	WP_004213077.1	hypothetical protein	-
I5G01_RS26120	60318..60611	-	294	WP_004213076.1	hypothetical protein	-
I5G01_RS26125	60627..61760	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
I5G01_RS26130	62368..62598	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I5G01_RS26135	62595..63038	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
I5G01_RS26140	63052..63468	-	417	Protein_68	restriction endonuclease	-
I5G01_RS26145	63472..63765	-	294	Protein_69	transposase	-
I5G01_RS26150	64182..64818	+	637	Protein_70	mucoid phenotype A regulator RmpA	-
I5G01_RS26155	65333..65739	-	407	Protein_71	GAF domain-containing protein	-
I5G01_RS26160	65830..66750	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
I5G01_RS26165	66799..67290	-	492	WP_011251327.1	DM13 domain-containing protein	-
I5G01_RS26170	67353..67628	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..204701	204701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T182544 WP_004213072.1 NZ_CP065402:62595-63038 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T182544 NZ_CP086490:1907581-1907832 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT182544 WP_004213073.1 NZ_CP065402:62368-62598 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT182544 NZ_CP086490:1907829-1908083 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620