Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 42555..43465 Replicon plasmid pn-1
Accession NZ_CP065402
Organism Klebsiella pneumoniae strain KPNSP-1

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag I5G01_RS26030 Protein ID WP_004026354.1
Coordinates 42995..43465 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag I5G01_RS26025 Protein ID WP_004026357.1
Coordinates 42555..42998 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5G01_RS25995 37573..38094 + 522 WP_004213635.1 sigma-70 family RNA polymerase sigma factor -
I5G01_RS26000 38091..39044 + 954 Protein_40 fec operon regulator FecR -
I5G01_RS26005 39131..41257 + 2127 WP_004213640.1 TonB-dependent receptor family protein -
I5G01_RS26010 41332..41487 + 156 WP_004213642.1 hypothetical protein -
I5G01_RS26015 41632..41853 + 222 WP_004213643.1 DUF2188 domain-containing protein -
I5G01_RS26020 41951..42454 + 504 Protein_44 DUF4113 domain-containing protein -
I5G01_RS26025 42555..42998 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
I5G01_RS26030 42995..43465 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
I5G01_RS26035 43920..44126 - 207 WP_004902422.1 hypothetical protein -
I5G01_RS26040 44722..44982 - 261 WP_004026352.1 hypothetical protein -
I5G01_RS26045 45553..46734 - 1182 WP_004883380.1 IS481 family transposase -
I5G01_RS26050 46806..46964 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
I5G01_RS26055 47183..48151 - 969 WP_004225014.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / rmpA2 / iutA / iucD / iucC / iucB / iucA 1..204701 204701


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T182543 WP_004026354.1 NZ_CP065402:42995-43465 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T182543 NZ_CP086490:1077623-1077958 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT182543 WP_004026357.1 NZ_CP065402:42555-42998 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT182543 NZ_CP086490:1077375-1077623 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References