Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5228422..5229047 Replicon chromosome
Accession NZ_CP065401
Organism Klebsiella pneumoniae strain KPNSP-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I5G01_RS25435 Protein ID WP_002882817.1
Coordinates 5228422..5228805 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I5G01_RS25440 Protein ID WP_004150355.1
Coordinates 5228805..5229047 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5G01_RS25420 5225788..5226690 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
I5G01_RS25425 5226687..5227322 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
I5G01_RS25430 5227319..5228248 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I5G01_RS25435 5228422..5228805 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I5G01_RS25440 5228805..5229047 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I5G01_RS25445 5229252..5230169 + 918 WP_004901674.1 alpha/beta hydrolase -
I5G01_RS25450 5230183..5231124 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I5G01_RS25455 5231169..5231606 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I5G01_RS25460 5231603..5232463 - 861 WP_002882807.1 virulence factor BrkB family protein -
I5G01_RS25465 5232457..5233056 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182542 WP_002882817.1 NZ_CP065401:c5228805-5228422 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182542 NZ_CP086490:933274-933681 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182542 WP_004150355.1 NZ_CP065401:c5229047-5228805 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182542 NZ_CP086490:933027-933293 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References