Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4007853..4008472 Replicon chromosome
Accession NZ_CP065401
Organism Klebsiella pneumoniae strain KPNSP-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I5G01_RS19675 Protein ID WP_002892050.1
Coordinates 4008254..4008472 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I5G01_RS19670 Protein ID WP_002892066.1
Coordinates 4007853..4008227 (+) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5G01_RS19660 4003005..4004198 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I5G01_RS19665 4004221..4007367 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
I5G01_RS19670 4007853..4008227 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I5G01_RS19675 4008254..4008472 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I5G01_RS19680 4008631..4009197 + 567 WP_002892030.1 maltose O-acetyltransferase -
I5G01_RS19685 4009169..4009309 - 141 WP_004147370.1 hypothetical protein -
I5G01_RS19690 4009330..4009800 + 471 WP_002892026.1 YlaC family protein -
I5G01_RS19695 4009775..4011226 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
I5G01_RS19700 4011327..4012025 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
I5G01_RS19705 4012022..4012162 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I5G01_RS19710 4012162..4012425 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(13-69)

Antitoxin

(1-116)


Sequences


Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182539 WP_002892050.1 NZ_CP065401:4008254-4008472 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T182539 NZ_CP086490:655568-655882 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT182539 WP_002892066.1 NZ_CP065401:4007853-4008227 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT182539 NZ_CP086490:655879-656295 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References