Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 201270..202009 Replicon chromosome
Accession NZ_CP065401
Organism Klebsiella pneumoniae strain KPNSP-1

Toxin (Protein)


Gene name tacT Uniprot ID A0A4U9WP91
Locus tag I5G01_RS00980 Protein ID WP_004901283.1
Coordinates 201524..202009 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag I5G01_RS00975 Protein ID WP_003026799.1
Coordinates 201270..201536 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5G01_RS00960 196774..198843 + 2070 WP_004901297.1 glycine--tRNA ligase subunit beta -
I5G01_RS00965 199139..200508 + 1370 WP_087661561.1 IS3 family transposase -
I5G01_RS00970 200709..201137 + 429 WP_004901287.1 GFA family protein -
I5G01_RS00975 201270..201536 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
I5G01_RS00980 201524..202009 + 486 WP_004901283.1 GNAT family N-acetyltransferase Toxin
I5G01_RS00985 202334..203380 - 1047 WP_004173931.1 IS481-like element ISKpn28 family transposase -
I5G01_RS00990 203454..203606 + 153 WP_002922102.1 type I toxin-antitoxin system toxin HokA -
I5G01_RS00995 203908..205527 + 1620 WP_004901278.1 ATP-binding cassette domain-containing protein -
I5G01_RS01000 205626..205838 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
I5G01_RS01005 206090..206380 - 291 WP_002921931.1 HTH-type transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 199783..203380 3597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17594.32 Da        Isoelectric Point: 10.1788

>T182528 WP_004901283.1 NZ_CP065401:201524-202009 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLNQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Download         Length: 486 bp

>T182528 NZ_CP086481:69724-69876 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT182528 WP_003026799.1 NZ_CP065401:201270-201536 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT182528 NZ_CP086481:c69674-69612 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9WP91

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References