Detailed information of TA system TA182527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1232852..1233522
• Replicon: chromosome
• Accession: NZ_CP065400
• Organism: Brucella sp. BO2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A6M2XLD2
• Locus tag: I5770_RS13765
• Protein ID: WP_005934647.1
• Coordinates: 1232852..1233271 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: I5770_RS13770
• Protein ID: WP_009363659.1
• Coordinates: 1233268..1233522 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5770_RS13720	1228331..1228462	-	132	Protein_1127	recombinase family protein	-
I5770_RS13725	1228638..1228814	+	177	WP_096222004.1	transcriptional regulator	-
I5770_RS13730	1228811..1229269	+	459	WP_025199226.1	helix-turn-helix domain-containing protein	-
I5770_RS13735	1229307..1229675	-	369	WP_009363656.1	conjugal transfer transcriptional regulator TraJ	-
I5770_RS13740	1229678..1229959	-	282	WP_025199227.1	hypothetical protein	-
I5770_RS13745	1229971..1230213	-	243	WP_009363657.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
I5770_RS13750	1230282..1231754	-	1473	WP_025199228.1	P-type conjugative transfer protein TrbL	-
I5770_RS13755	1231756..1231914	-	159	WP_156797749.1	hypothetical protein	-
I5770_RS13760	1231925..1232695	-	771	WP_009363658.1	P-type conjugative transfer protein TrbJ	-
I5770_RS13765	1232852..1233271	-	420	WP_005934647.1	type II toxin-antitoxin system VapC family toxin	Toxin
I5770_RS13770	1233268..1233522	-	255	WP_009363659.1	Arc family DNA-binding protein	Antitoxin
I5770_RS13775	1234626..1235378	-	753	WP_009363660.1	hypothetical protein	-
I5770_RS13780	1235365..1236243	-	879	WP_009363661.1	helicase RepA family protein	-
I5770_RS13785	1236246..1236470	-	225	WP_009363662.1	AlpA family phage regulatory protein	-
I5770_RS13790	1236556..1237557	+	1002	WP_009363663.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1228145..1239163	11018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14897.11 Da
• Isoelectric Point: 5.1890

>T182527 WP_005934647.1 NZ_CP065400:c1233271-1232852 [Brucella sp. BO2]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVAETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAVKARTAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Nucleotide

• Length: 420 bp

>T182527 NZ_CP086481:2255-2533 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9509.81 Da
• Isoelectric Point: 5.2076

>AT182527 WP_009363659.1 NZ_CP065400:c1233522-1233268 [Brucella sp. BO2]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSEERIRMGDALAELGRRVGLTNDDFAVLDQVRDKVPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT182527 NZ_CP086481:1986-2255 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A6M2XLD2