Detailed information of TA system TA182526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 92101..92771
• Replicon: chromosome
• Accession: NZ_CP065399
• Organism: Brucella sp. BO2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I5770_RS00475
• Protein ID: WP_008504608.1
• Coordinates: 92367..92771 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D1CXH4
• Locus tag: I5770_RS00470
• Protein ID: WP_008504606.1
• Coordinates: 92101..92370 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5770_RS00450	89120..89317	+	198	WP_008504596.1	DUF3008 family protein	-
I5770_RS00455	89362..89775	-	414	WP_009364413.1	hypothetical protein	-
I5770_RS00460	90047..91018	-	972	WP_025199540.1	extensin family protein	-
I5770_RS00465	91353..91970	+	618	WP_002967582.1	30S ribosomal protein S4	-
I5770_RS00470	92101..92370	+	270	WP_008504606.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I5770_RS00475	92367..92771	+	405	WP_008504608.1	type II toxin-antitoxin system VapC family toxin	Toxin
I5770_RS00480	93066..93947	+	882	WP_008504610.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
I5770_RS00485	93934..94761	+	828	WP_008504612.1	inositol monophosphatase family protein	-
I5770_RS00490	94815..96080	-	1266	WP_025199539.1	multidrug effflux MFS transporter	-
I5770_RS00495	96351..96683	-	333	WP_009364410.1	Grx4 family monothiol glutaredoxin	-
I5770_RS00500	96865..97101	-	237	WP_008505519.1	BolA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14365.66 Da
• Isoelectric Point: 5.6366

>T182526 WP_008504608.1 NZ_CP065399:92367-92771 [Brucella sp. BO2]
MSKLYMLDTNIVSDLARNPHGPVAKHIARVGADAICVSIITACELRYGCAKKGSPRLLAQIEAILDSIEILPLDVPADTE
YGGIRAELEADGKPIGPNDFLIAAHAYALGAVLVTANVGEFTRIRKLAVENWLN

Nucleotide

• Length: 405 bp

>T182526 NZ_CP086480:c4346291-4346118 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10046.62 Da
• Isoelectric Point: 7.2059

>AT182526 WP_008504606.1 NZ_CP065399:92101-92370 [Brucella sp. BO2]
MAHARRIEPTPAREAKLFRNNKSQAVRIPADFEFPGNRVLIHREGDKLIIEPVRRKNLLEVLAGLKPLGPEDAFPDIDDT
ALPLKDVDL

Nucleotide

• Length: 270 bp

>AT182526 NZ_CP086480:c4346615-4346319 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7L7MKG1