Detailed information of TA system TA182524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 61612..62213
• Replicon: plasmid p1
• Accession: NZ_CP065398
• Organism: Bifidobacterium longum strain I2-2-3

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: I3K78_RS10165
• Protein ID: WP_164907608.1
• Coordinates: 61612..61911 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I3K78_RS10170
• Protein ID: WP_077384693.1
• Coordinates: 61923..62213 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K78_RS10135 (I3K78_10125)	57078..57614	-	537	WP_007058063.1	hypothetical protein	-
I3K78_RS10140 (I3K78_10130)	57604..58308	-	705	WP_249159932.1	hypothetical protein	-
I3K78_RS10145 (I3K78_10135)	58673..58933	-	261	WP_007055861.1	hypothetical protein	-
I3K78_RS10150 (I3K78_10140)	59059..59643	+	585	WP_007055860.1	helix-turn-helix transcriptional regulator	-
I3K78_RS10155 (I3K78_10145)	59715..60161	+	447	WP_101026260.1	ImmA/IrrE family metallo-endopeptidase	-
I3K78_RS10160 (I3K78_10150)	60180..61493	+	1314	WP_212106171.1	zinc ribbon domain-containing protein	-
I3K78_RS10165 (I3K78_10155)	61612..61911	+	300	WP_164907608.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3K78_RS10170 (I3K78_10160)	61923..62213	+	291	WP_077384693.1	putative addiction module antidote protein	Antitoxin
I3K78_RS10175 (I3K78_10165)	62247..62708	-	462	WP_117768727.1	DNA N-6-adenine-methyltransferase	-
I3K78_RS10180 (I3K78_10170)	62708..63502	-	795	WP_212106172.1	hypothetical protein	-
I3K78_RS10185 (I3K78_10175)	63670..64101	-	432	WP_249159933.1	hypothetical protein	-
I3K78_RS10190 (I3K78_10180)	64079..64333	-	255	WP_249159934.1	hypothetical protein	-
I3K78_RS10195 (I3K78_10185)	64781..66223	-	1443	WP_117761133.1	RNA-guided endonuclease TnpB family protein	-
I3K78_RS10200 (I3K78_10190)	66334..66657	-	324	WP_225090436.1	hypothetical protein	-
I3K78_RS10205 (I3K78_10195)	66660..67067	-	408	WP_101014366.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..198121	198121
	-	flank	IS/Tn	-	-	64781..66337	1556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-97)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11713.55 Da
• Isoelectric Point: 10.0166

>T182524 WP_164907608.1 NZ_CP065398:61612-61911 [Bifidobacterium longum]
MRFKRTSEFLEWLRKLKDRDAKAKIEAHLDYCELKGSLLGDTKPVGDSIYEMRFHFGPGYRIYYARSGSEIYLLLIGGDK
RKQQADIKKAKKLFREYSE

Nucleotide

• Length: 300 bp

>T182524 NZ_CP086480:c4128021-4127734 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10660.96 Da
• Isoelectric Point: 4.6151

>AT182524 WP_077384693.1 NZ_CP065398:61923-62213 [Bifidobacterium longum]
MTKKYQDYDTSEFLETEDDIARYLNAVAEYNDPEMFQTALGKVAKARGMSSIAKEANVSRESLYRSLSAEGNPSFQTISK
VLASMGLRLTIQPAEA

Nucleotide

• Length: 291 bp

>AT182524 NZ_CP086480:c4127747-4127445 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA