Detailed information of TA system TA182522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 562056..562545
• Replicon: chromosome
• Accession: NZ_CP065397
• Organism: Bifidobacterium longum strain I2-2-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: I3K78_RS02390
• Protein ID: WP_229773994.1
• Coordinates: 562318..562545 (+)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A4V2N072
• Locus tag: I3K78_RS02385
• Protein ID: WP_032744823.1
• Coordinates: 562056..562292 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K78_RS02365 (I3K78_02355)	558209..559396	+	1188	WP_174774172.1	carboxylate--amine ligase	-
I3K78_RS02370 (I3K78_02360)	559424..560749	+	1326	WP_234932960.1	MFS transporter	-
I3K78_RS02385 (I3K78_02375)	562056..562292	+	237	WP_032744823.1	ribbon-helix-helix domain-containing protein	Antitoxin
I3K78_RS02390 (I3K78_02380)	562318..562545	+	228	WP_229773994.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3K78_RS02395 (I3K78_02385)	562896..563558	-	663	WP_003828427.1	tRNA (cytidine(34)-2'-O)-methyltransferase	-
I3K78_RS02400 (I3K78_02390)	563579..564535	+	957	WP_212105731.1	A/G-specific adenine glycosylase	-
I3K78_RS02405 (I3K78_02395)	564600..565322	+	723	WP_060620046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-72)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8653.22 Da
• Isoelectric Point: 10.1818

>T182522 WP_229773994.1 NZ_CP065397:562318-562545 [Bifidobacterium longum]
MKKLDRNVAKRIIAKLREISQLEDPRSTGKALAGNLAGLWRYRVGDYRIVCDIEDEVLLILVIDVAHRSKVYKRC

Nucleotide

• Length: 228 bp

>T182522 NZ_CP086480:c3786625-3786227 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8928.15 Da
• Isoelectric Point: 4.2821

>AT182522 WP_032744823.1 NZ_CP065397:562056-562292 [Bifidobacterium longum]
MATVTAALRMPVELAARYDCLAKATGRTKTFYMNEALTESIDRFEYEYGIMKKVEDWRAGRLETVTLDELEESLGLED

Nucleotide

• Length: 237 bp

>AT182522 NZ_CP086480:c3786921-3786628 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A4V2N072