Detailed information of TA system TA182521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 189066..189667
• Replicon: plasmid p1
• Accession: NZ_CP065396
• Organism: Bifidobacterium longum strain K2-21-4

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: I3K77_RS10895
• Protein ID: WP_164907608.1
• Coordinates: 189368..189667 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I3K77_RS10890
• Protein ID: WP_077384693.1
• Coordinates: 189066..189356 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K77_RS10855 (I3K77_10850)	184212..184619	+	408	WP_101014366.1	hypothetical protein	-
I3K77_RS10860 (I3K77_10855)	184622..184945	+	324	WP_225090436.1	hypothetical protein	-
I3K77_RS10865 (I3K77_10860)	185056..186498	+	1443	WP_117761133.1	RNA-guided endonuclease TnpB family protein	-
I3K77_RS10870 (I3K77_10865)	186946..187200	+	255	WP_249159934.1	hypothetical protein	-
I3K77_RS10875 (I3K77_10870)	187178..187609	+	432	WP_249159933.1	hypothetical protein	-
I3K77_RS10880 (I3K77_10875)	187804..188571	+	768	WP_249162358.1	hypothetical protein	-
I3K77_RS10885 (I3K77_10880)	188571..189032	+	462	WP_117768727.1	DNA N-6-adenine-methyltransferase	-
I3K77_RS10890 (I3K77_10885)	189066..189356	-	291	WP_077384693.1	putative addiction module antidote protein	Antitoxin
I3K77_RS10895 (I3K77_10890)	189368..189667	-	300	WP_164907608.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3K77_RS10900 (I3K77_10895)	189786..191099	-	1314	WP_212106171.1	zinc ribbon domain-containing protein	-
I3K77_RS10905 (I3K77_10900)	191118..191564	-	447	WP_101026260.1	ImmA/IrrE family metallo-endopeptidase	-
I3K77_RS10910 (I3K77_10905)	191636..192220	-	585	WP_007055860.1	helix-turn-helix transcriptional regulator	-
I3K77_RS10915 (I3K77_10910)	192346..192606	+	261	WP_007055861.1	hypothetical protein	-
I3K77_RS10920 (I3K77_10915)	192971..193675	+	705	WP_249159932.1	hypothetical protein	-
I3K77_RS10925 (I3K77_10920)	193665..194201	+	537	WP_007058063.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..198121	198121
	-	flank	IS/Tn	-	-	184942..186498	1556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-97)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11713.55 Da
• Isoelectric Point: 10.0166

>T182521 WP_164907608.1 NZ_CP065396:c189667-189368 [Bifidobacterium longum]
MRFKRTSEFLEWLRKLKDRDAKAKIEAHLDYCELKGSLLGDTKPVGDSIYEMRFHFGPGYRIYYARSGSEIYLLLIGGDK
RKQQADIKKAKKLFREYSE

Nucleotide

• Length: 300 bp

>T182521 NZ_CP086480:3573996-3574214 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10660.96 Da
• Isoelectric Point: 4.6151

>AT182521 WP_077384693.1 NZ_CP065396:c189356-189066 [Bifidobacterium longum]
MTKKYQDYDTSEFLETEDDIARYLNAVAEYNDPEMFQTALGKVAKARGMSSIAKEANVSRESLYRSLSAEGNPSFQTISK
VLASMGLRLTIQPAEA

Nucleotide

• Length: 291 bp

>AT182521 NZ_CP086480:3573596-3573970 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG