Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/PIN-RelB
Location 1370234..1371114 Replicon chromosome
Accession NZ_CP065395
Organism Bifidobacterium longum strain K2-21-4

Toxin (Protein)


Gene name relE Uniprot ID F8APN2
Locus tag I3K77_RS05860 Protein ID WP_007052625.1
Coordinates 1370234..1370725 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A4R0V8T5
Locus tag I3K77_RS05865 Protein ID WP_013410773.1
Coordinates 1370722..1371114 (-) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3K77_RS05840 (I3K77_05835) 1365287..1366549 + 1263 WP_223616533.1 histidine kinase -
I3K77_RS05845 (I3K77_05840) 1366584..1367309 + 726 WP_007056206.1 response regulator transcription factor -
I3K77_RS05850 (I3K77_05845) 1367349..1367990 + 642 WP_007052627.1 GNAT family N-acetyltransferase -
I3K77_RS05855 (I3K77_05850) 1368079..1370184 + 2106 WP_007052626.1 DUF262 domain-containing protein -
I3K77_RS05860 (I3K77_05855) 1370234..1370725 - 492 WP_007052625.1 type II toxin-antitoxin system VapC family toxin Toxin
I3K77_RS05865 (I3K77_05860) 1370722..1371114 - 393 WP_013410773.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
I3K77_RS05870 (I3K77_05865) 1371260..1373959 - 2700 WP_007054466.1 aconitate hydratase AcnA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-146)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-53)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 18531.70 Da        Isoelectric Point: 6.2348

>T182519 WP_007052625.1 NZ_CP065395:c1370725-1370234 [Bifidobacterium longum]
MRQSKQVSILCDTNILIYYMLCTPRFIKDVMKLFERCVEREVKLSCASLSLKDVFYITPERFKERFMNDESMVVATTAKL
ILPEVAWSGISKLKELMTIVPIGAEECELAQSLRHAHNDFEDDLIIAAAQQSGATYVVTYDKKLAKHFPEYCKTPAEIVA
ALA

Download         Length: 492 bp

>T182519 NZ_CP086480:2755167-2755274 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 15205.33 Da        Isoelectric Point: 5.4043

>AT182519 WP_013410773.1 NZ_CP065395:c1371114-1370722 [Bifidobacterium longum]
MTMVQMNVRIDAKLKNEVEEVLKGKGVSVSDVIRSLWLYIADRKEVPALETTAEEQAREEEKQRKLKLIREGAGYVHKEL
VKAGLISEDTDLMEGLTYKQFRDEIADERLKEYRHQSDYARYLEKERSLS

Download         Length: 393 bp

>AT182519 NZ_CP086480:c2755120-2755054 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F8APN2

Antitoxin

Source ID Structure
AlphaFold DB A0A4R0V8T5

References