Detailed information of TA system TA182518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 562055..562544
• Replicon: chromosome
• Accession: NZ_CP065395
• Organism: Bifidobacterium longum strain K2-21-4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: I3K77_RS02380
• Protein ID: WP_229773994.1
• Coordinates: 562317..562544 (+)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A4V2N072
• Locus tag: I3K77_RS02375
• Protein ID: WP_032744823.1
• Coordinates: 562055..562291 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K77_RS02355 (I3K77_02350)	558208..559395	+	1188	WP_174774172.1	carboxylate--amine ligase	-
I3K77_RS02360 (I3K77_02355)	559423..560748	+	1326	WP_234932960.1	MFS transporter	-
I3K77_RS02375 (I3K77_02370)	562055..562291	+	237	WP_032744823.1	ribbon-helix-helix domain-containing protein	Antitoxin
I3K77_RS02380 (I3K77_02375)	562317..562544	+	228	WP_229773994.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I3K77_RS02385 (I3K77_02380)	562895..563557	-	663	WP_003828427.1	tRNA (cytidine(34)-2'-O)-methyltransferase	-
I3K77_RS02390 (I3K77_02385)	563578..564534	+	957	WP_212105731.1	A/G-specific adenine glycosylase	-
I3K77_RS02395 (I3K77_02390)	564599..565321	+	723	WP_060620046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	553944..562544	8600

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-72)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8653.22 Da
• Isoelectric Point: 10.1818

>T182518 WP_229773994.1 NZ_CP065395:562317-562544 [Bifidobacterium longum]
MKKLDRNVAKRIIAKLREISQLEDPRSTGKALAGNLAGLWRYRVGDYRIVCDIEDEVLLILVIDVAHRSKVYKRC

Nucleotide

• Length: 228 bp

>T182518 NZ_CP086480:c2542205-2542029 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8928.15 Da
• Isoelectric Point: 4.2821

>AT182518 WP_032744823.1 NZ_CP065395:562055-562291 [Bifidobacterium longum]
MATVTAALRMPVELAARYDCLAKATGRTKTFYMNEALTESIDRFEYEYGIMKKVEDWRAGRLETVTLDELEESLGLED

Nucleotide

• Length: 237 bp

>AT182518 NZ_CP086480:c2541983-2541567 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4V2N072