Detailed information of TA system TA182517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2012646..2014195
• Replicon: chromosome
• Accession: NZ_CP065386
• Organism: Glaesserella parasuis strain LHDR_HPS_1_2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: I5Z97_RS09875
• Protein ID: WP_075630951.1
• Coordinates: 2012646..2013962 (-)
• Length: 439 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: U4SAZ6
• Locus tag: I5Z97_RS09880
• Protein ID: WP_010786623.1
• Coordinates: 2013956..2014195 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5Z97_RS09855	2009600..2010472	+	873	WP_075630953.1	LysR family transcriptional regulator	-
I5Z97_RS09860	2010533..2011153	-	621	WP_075630952.1	DsbA family protein	-
I5Z97_RS09865	2011262..2011498	+	237	WP_196980236.1	GntR family transcriptional regulator	-
I5Z97_RS09870	2011506..2012642	+	1137	WP_196980237.1	PLP-dependent aminotransferase family protein	-
I5Z97_RS09875	2012646..2013962	-	1317	WP_075630951.1	type II toxin-antitoxin system HipA family toxin	Toxin
I5Z97_RS09880	2013956..2014195	-	240	WP_010786623.1	helix-turn-helix domain-containing protein	Antitoxin
I5Z97_RS09885	2014343..2015170	-	828	WP_021116417.1	alpha/beta hydrolase	-
I5Z97_RS09890	2015301..2016746	+	1446	WP_075630950.1	metalloprotease TldD	-
I5Z97_RS09895	2016974..2018071	+	1098	WP_196980238.1	ISAs1 family transposase	-
I5Z97_RS09900	2018215..2018625	+	411	WP_010786620.1	preprotein translocase subunit SecE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-104)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-66)

Sequences

Toxin

Protein

• Length: 439 a.a.
• Molecular weight: 49995.24 Da
• Isoelectric Point: 7.6047

>T182517 WP_075630951.1 NZ_CP065386:c2013962-2012646 [Glaesserella parasuis]
MVMRLRVLMNGEMVGYFDRTLSGSHHFTYAESWLNSPLARPISLSMPLQRATYQGKEVINFFDNLLPDNPQIRARIATRF
NAETQQPFDLLAKIGRDAAGAITLLGEDEQIPNHRIIQATELDESEMAKILQSYQYNAPLGMIDNNQDFRISIAGAQEKT
ALLWHNGKWKLPHGLTPTTHIFKLPMGEIRTPKHLLDLSKSVDNELVCLRLFSAFNIPSANVELLEMNDIRALAVERFDR
RLSQDGSYFLRLPYEDFCQAFGLSPTQKYESDGGIGIGDMMNKLLYSSNSNDRRQFMQTQFLFWLLAAIDGHAKNFSLAI
EKQGRYHLSPVYDVLSAYPMLGGKGWHHSELQMAMSLRSTQKGKKWHWSKIYAEHFYATAKFVGFSSSDMKQVIEQICDT
VQETIDRVATTLPKQIDENVRDQIFTGVIKQVQKVSMK

Nucleotide

• Length: 1317 bp

>T182517 NZ_CP086480:2455047-2456369 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9059.47 Da
• Isoelectric Point: 9.7684

>AT182517 WP_010786623.1 NZ_CP065386:c2014195-2013956 [Glaesserella parasuis]
MLITTPDMLGNSLRDIRKQKKLSQMQIANQVALRQATISTFEKKTDKATIETLFKLLSGLGYELHIQPRPEKTEGNDEW

Nucleotide

• Length: 240 bp

>AT182517 NZ_CP086480:2454781-2455047 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: U4SAZ6