Detailed information of TA system TA182516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1990010..1990616
• Replicon: chromosome
• Accession: NZ_CP065386
• Organism: Glaesserella parasuis strain LHDR_HPS_1_2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: I5Z97_RS09745
• Protein ID: WP_021114171.1
• Coordinates: 1990010..1990327 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I5Z97_RS09750
• Protein ID: WP_021114170.1
• Coordinates: 1990329..1990616 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5Z97_RS09720	1986830..1987687	-	858	WP_075630627.1	DUF1837 domain-containing protein	-
I5Z97_RS09725	1987738..1987929	-	192	Protein_1874	ATPase	-
I5Z97_RS09730	1988068..1988868	-	801	WP_075630626.1	efflux RND transporter permease subunit	-
I5Z97_RS09735	1988913..1989455	-	543	WP_043896593.1	efflux RND transporter permease subunit	-
I5Z97_RS09740	1989395..1989793	-	399	WP_075630625.1	efflux RND transporter permease subunit	-
I5Z97_RS09745	1990010..1990327	+	318	WP_021114171.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5Z97_RS09750	1990329..1990616	+	288	WP_021114170.1	putative addiction module antidote protein	Antitoxin
I5Z97_RS09755	1990883..1991841	-	959	Protein_1880	efflux RND transporter periplasmic adaptor subunit	-
I5Z97_RS09760	1992036..1993172	-	1137	WP_021119330.1	restriction endonuclease subunit S	-
I5Z97_RS09765	1993193..1995235	-	2043	WP_196980234.1	N-6 DNA methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Uncharacterized protein HI1420 (3-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11965.65 Da
• Isoelectric Point: 9.3816

>T182516 WP_021114171.1 NZ_CP065386:1990010-1990327 [Glaesserella parasuis]
MNVIETTLIFDEWLDNLKDLRAKIQIVTRIRRAENGNFGDHKPLPNTGGVSEIRLDIGKGYRIYYGQIGEITYILTNGGD
KSSQQADIEKAKALFVQIKQQKKEQ

Nucleotide

• Length: 318 bp

>T182516 NZ_CP086480:2144417-2144520 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10484.04 Da
• Isoelectric Point: 5.0489

>AT182516 WP_021114170.1 NZ_CP065386:1990329-1990616 [Glaesserella parasuis]
MIETNRFDIVKYLDSDEAIAIYLTQIAETGDSNDFIVALGDVARAKGIAEIAQKTGLARESIYKALTSGSKPRFDTIMRI
MNALNLKPQYAINLS

Nucleotide

• Length: 288 bp

>AT182516 NZ_CP086480:c2144558-2144287 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT