Detailed information of TA system TA182515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1318400..1319177
• Replicon: chromosome
• Accession: NZ_CP065386
• Organism: Glaesserella parasuis strain LHDR_HPS_1_2

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: U4SQ43
• Locus tag: I5Z97_RS06385
• Protein ID: WP_021116693.1
• Coordinates: 1318677..1319177 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: U4SPD7
• Locus tag: I5Z97_RS06380
• Protein ID: WP_005710653.1
• Coordinates: 1318400..1318687 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5Z97_RS06360	1314768..1315268	-	501	WP_016527798.1	CvpA family protein	-
I5Z97_RS06365	1315372..1316949	-	1578	WP_160421808.1	FAD-binding protein	-
I5Z97_RS06370	1317124..1317861	-	738	WP_021112465.1	peptidoglycan editing factor PgeF	-
I5Z97_RS06375	1317865..1318254	-	390	WP_021113660.1	RidA family protein	-
I5Z97_RS06380	1318400..1318687	+	288	WP_005710653.1	DUF1778 domain-containing protein	Antitoxin
I5Z97_RS06385	1318677..1319177	+	501	WP_021116693.1	hypothetical protein	Toxin
I5Z97_RS06390	1319205..1320206	+	1002	WP_010786647.1	nucleoid-associated protein YejK	-
I5Z97_RS06395	1320267..1320602	+	336	WP_016527793.1	outer membrane protein assembly factor BamE	-
I5Z97_RS06400	1320756..1321871	+	1116	WP_016527792.1	anhydro-N-acetylmuramic acid kinase	-
I5Z97_RS06405	1321883..1322797	+	915	WP_021113663.1	N-acetylmuramic acid 6-phosphate etherase	-
I5Z97_RS06410	1322839..1323153	+	315	WP_005710664.1	YqcC family protein	-
I5Z97_RS06415	1323150..1323878	+	729	WP_021112446.1	tRNA pseudouridine(65) synthase TruC	-
I5Z97_RS06420	1323896..1324051	+	156	WP_021116533.1	YoaH family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 19113.26 Da
• Isoelectric Point: 9.0168

>T182515 WP_021116693.1 NZ_CP065386:1318677-1319177 [Glaesserella parasuis]
MKSKFIEEPLTKHHQREAFDCGNLEMNRFFKQYARQSHEKGTSKTYISRHLDSQQIIGFYTITLSALDQQHLPELCKKRF
GYHPIPLFTLARLAVDIRYQKQDIGGMLLVKALKRCAIVAEQVGGIGLLIEAKDEDISRWYQSYGAIPLENMPLTLILLF
DTIKGL

Nucleotide

• Length: 501 bp

>T182515 NZ_CP086480:c1920891-1920514 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10917.47 Da
• Isoelectric Point: 6.4650

>AT182515 WP_005710653.1 NZ_CP065386:1318400-1318687 [Glaesserella parasuis]
MPQIANALNDRMSIRISTEDKQTLIRAVALTHTNITEFVLQRILPSAREIIQQYDNRQFSPQDLSLIMELLDNPPKANTK
LQQAAKLAKESYHEE

Nucleotide

• Length: 288 bp

>AT182515 NZ_CP086480:c1921349-1920981 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCACCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: U4SQ43

Antitoxin

• AlphaFold DB: U4SPD7